Glut3 Antibody (JA50-31)

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. Beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.

Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of SH-SY5Y cells with Glucose Transporter GLUT3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/5,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)Lane 2: RAW264.7 cell lysate (20 ug/Lane)Lane 3: C6 cell lysate (20 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/1,000 dilution.Lane 1: HepG2-si NT cell lysate (10 ug/Lane)Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 1 minute; ECL: merk4-20% SDS-PAGE gel.NBP2-66872 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow
• Clone: JA50-31
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Glut3 Antibody (JA50-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Glut3 aa 11-60 / 496. (SwissProt: P11169 Human; SwissProt: P32037 Mouse; SwissProt: Q07647 Rat)
• Localization: Cell membrane. Perikaryon. Cell projection.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC2A3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glut3 Antibody (JA50-31)

• FLJ90380
• Glut3
• GLUT-3
• GLUT3Glucose transporter type 3, brain
• SLC2A3
• solute carrier family 2 (facilitated glucose transporter), member 3
• solute carrier family 2, facilitated glucose transporter member 3

Background

Glucose is the major source of our energy and there are numerous isoforms of the glucose transporter in mammals, including Glut1, Glut2, Glut3, Glut4, Glut5, Glut6, Glut7, Glut8 and Glut9. The Glut5 gene located on the short arm of human chromosome 1 encodes a 501-amino acid facilitative glucose transporter. Glut5 mRNA is highly expressed in small intestine and to a lesser extent in kidney, skeletal muscle and adipose tissue. Glut5 plays a critical role in fructose absorption in the small intestine and its expression is highly induced when exposed to a fructose-enriched diet. Glut5 transporter expressed in human skeletal muscle is specifically localized to the plasma membrane, where it participates in regulating hexose transfer across the sarcolemma. Glut8, a novel glucose transporter-like protein, exhibits significant sequence similarity with the other members of sugar transporter family. Glut8 comprises 12 putative membrane-spanning helices and several conserved motifs, which are important for transport activity. In human tissues, Glut8 is predominantly expressed in testis and, to a lesser extent, in most other tissues including skeletal muscle, heart, small intestine and brain. In addition, the Glut8 glucose transport facilitator has a hormonally regulated testicular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut3 Antibody (NBP2-66872)(5)

Publications using NBP2-66872

• He Q, Ji L, Wang Y, Zhang Y et Al. Acetate enables metabolic fitness and cognitive performance during sleep disruption Cell Metab 2024-08-20 [PMID: 39163862]
• Wessendarp M, Watanabe-Chailland M, Liu S Et al. Role of GM-CSF in regulating metabolism and mitochondrial functions critical to macrophage proliferation Mitochondrion 2021-11-01 [PMID: 34740864] (FLOW, Mouse)
• Uddin MM, Ibrahim MMH, Aryal D, Briski KP Sex-dimorphic moderate hypoglycemia preconditioning effects on Hippocampal CA1 neuron bio-energetic and anti-oxidant function Mol. Cell. Biochem. 2020-08-10 [PMID: 32779041] (WB, Rat)
• Mori, S, Kurimoto, T Et al. Aqp9 Gene Deletion Enhances Retinal Ganglion Cell (RGC) Death and Dysfunction Induced by Optic Nerve Crush: Evidence that Aquaporin 9 Acts as an Astrocyte-to-Neuron Lactate Shuttle in Concert with Monocarboxylate Transporters To Support RGC Function and . Mol Neurobiol 2020-11-01 [PMID: 32748371] (ICC/IF, Human)
• Briski K, Mandal S Hindbrain lactoprivic regulation of hypothalamic neuron transactivation and gluco-regulatory neurotransmitter expression: Impact of antecedent insulin-induced hypoglycemia Neuropeptides 2019-08-01 [PMID: 31488323] (WB, Rat)

Bioinformatics

• Gene Symbol: SLC2A3