GluR1 [p Ser845] Antibody - Azide Free

Western Blot: GluR1 [p Ser845] Antibody [NB300-171] - Rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Mu, Rt, Hu, Pm
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GluR1 [p Ser845] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH. Accession # P19490
• Modification: p Ser845
• Localization: Cell Membrane
• Specificity: Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser845. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Simple Western 1:100
  • Western Blot 1:1000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24212670)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GluR1 [p Ser845] Antibody - Azide Free

• AMPA 1
• AMPA-selective glutamate receptor 1
• GluA1
• GLUH1
• GluR1
• gluR-1
• GLUR1gluR-A
• GLURA
• GluR-A
• gluR-K1
• glutamate receptor 1
• Glutamate receptor ionotropic, AMPA 1
• glutamate receptor, ionotropic, AMPA 1
• GRIA1
• HBGR1
• MGC133252

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The GRIA1 belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Each of the members (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. The receptor subunits encoded by each isoform vary in their signal transduction properties. The isoform presented here is the flop isoform. In situ hybridization experiments showed that human GRIA1 mRNA is present in granule and pyramidal cells in the hippocampal formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GluR1 Antibody (NB300-171)(1)

Publication using NB300-171

• Kim J, Kwon JT, Kim HS et al. Memory recall and modifications by activating neurons with elevated CREB. Nat Neurosci. 2013-11-10 [PMID: 24212670] (WB, Mouse)

FAQs for GluR1 Antibody (NB300-171). (Showing 1 - 1 of 1 FAQ).

1. Could you please provide the immunogen sequence for catalog number NB300-171 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived?
   • The immunogen for this one was taken from within the 30 amino acids that surround the serine 845.

Bioinformatics

• Gene Symbol: GRIA1
• Entrez:
  • 2890(Human)
  • 14810(Mouse)
  • 50592(Rat)
• Uniprot:
  • P19490(Rat)