DLG1 Antibody

Images

 
Western Blot: DLG1 Antibody [NB600-1229] - Analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton ...read more
Immunocytochemistry/ Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DLG1 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues V(115) L P S E R I S P Q V P N E V L G P E(133) of rat SAP97.
Localization
Presynaptic membrane.
Specificity
Detects SAP 97 from rat tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLG1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 140 kDa protein representing SAP 97 from rat brain extract. IHC: Staining of SAP 97 in rat hippocampus results in intense neuronal cell body and dendrite staining.
Publications
Read Publication using NB600-1229.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for DLG1 Antibody

  • discs, large homolog 1 (Drosophila)
  • disks large homolog 1
  • DKFZp761P0818
  • DKFZp781B0426
  • hDlg
  • large (Drosophila) homolog 1
  • presynaptic protein SAP97

Background

Synapse-associated protein (SAP) 97 is a 97 kDa membraneassociated synapse protein with extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal. SAP97 consists of three 90 amino acid PDZ domains at its amino terminus, followed by a Src homology (SH) 3 domain, and a region with homology to guanylate kinases (GK). In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP97 is recruited to the submembranous cortical cytoskeleton at cell-cell contact sites as part of the E-cadherin complex. Co-expression of SAP97 and K+ channel results in the colocalization of these proteins in large round intracellular aggregates. SAP 97 has been shown to have functional homology to Drosophila tumor suppressor protein (DlgA), a PSD 95 related protein involved in maintaining integrity of epithelial and neuromuscular junctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DLG1 Antibody (NB600-1229)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DLG1
Entrez