GILT/IFI30 Antibody (003) - Azide and BSA Free

Immunohistochemistry-Paraffin: GILT/IFI30 Antibody (003) [NBP2-90133] - Immunochemical staining of human IFI30 in human lung with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: GILT/IFI30 Antibody (003) [NBP2-90133] - Immunochemical staining of human IFI30 in human tonsil with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Immunocytochemistry/Immunofluorescence: GILT/IFI30 Antibody (003) [NBP2-90133] - Immunofluorescence staining of IFI30 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90133 antibody (dilution ratio 1:30) at 4? overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: 003
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GILT/IFI30 Antibody (003) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human GILT/IFI30 (Uniprot#: P13284; Met1-Lys243).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFI30
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry-Paraffin 1:10000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GILT/IFI30 Antibody (003) - Azide and BSA Free

• EC 1.8
• Gamma-interferon-inducible protein IP-30
• GILT
• GILTgamma-interferon-inducible lysosomal thiol reductase
• IFI30
• IFI-30
• interferon, gamma-inducible protein 30
• IP30
• IP30interferon gamma-inducible protein 30 preproprotein
• Legumaturain
• MGC32056

Background

IFI30 is encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFI30