TTF2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TTF2 Antibody [NBP1-32842] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: TTF2 Antibody [NBP1-32842] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.

Immunoprecipitation: TTF2 Antibody [NBP1-32842] - TTF2 Antibody immunoprecipitates TTF2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TTF2 protein by 2 ug of TTF2 antibody on 7.5% SDS-PAGE. The immunoprecipitated TTF2 protein was detected by TTF2 Antibody diluted at 1:1000.

Western Blot: TTF2 Antibody [NBP1-32842] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TTF2 antibody (NBP1-32842) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TTF2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TTF2 Antibody - BSA Free (NBP1-32842) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TTF2. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TTF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TTF2 Antibody - BSA Free

• EC 3.6.1
• EC 3.6.4.-
• F2
• HuF2lodestar protein
• human factor 2
• Lodestar homolog
• RNA polymerase II termination factor
• Transcription release factor 2
• transcription termination factor 2
• transcription termination factor, RNA polymerase II

Background

This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TTF2