GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free

Western Blot: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-77035] - Crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1:500 dilution.

Immunohistochemistry: GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free [NBP1-77035] - Immunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 ug/mL.

Immunohistochemistry: GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free [NBP1-77035] - Immunohistochemistry of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free [NBP1-77035] - Immunofluorescence of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 ug/ml. Green: GFR alpha 3 antibody Red: Phylloidin staining Blue: DAPI staining

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free (NBP1-77035) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid peptide near the center of murine GFR alpha 3. The immunogen is located within the last 50 amino acids of GFR alpha 3. Amino Acid Squence: RFHSQLFSQDWPHPT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GFRA3
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5 ug/ml
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  GFR alpha-3/GDNF R alpha-3 Peptide (NBP1-77035PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free

• GDNF family receptor alpha 3
• GDNF family receptor alpha-3
• GDNF R alpha-3
• GDNF receptor alpha-3
• GDNFR-alpha-3
• GFR alpha3
• GFR alpha-3
• GFRA3
• GFRa-3
• GFR-alpha-3
• glial cell line-derived neurotrophic factor receptor alpha-3
• GPI-linked receptor

Background

Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRalpha and Ret receptor tyrosine kinase GFRbeta. Following the findings of GFRalpha-1 and -2, a novel receptor in GFRalpha family was identified very recently from human and mouse and designated GFRalpha-3. GFRalpha-3 binds persephin, thus, persephin, GFRalpha-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GFRA3
• Entrez:
  • 2676(Human)
  • 14587(Mouse)
• Uniprot:
  • O35118(Mouse)