CX3CL1/Fractalkine Antibody [Unconjugated]

Images

 
CX3CL1/Fractalkine was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal ...read more
Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

CX3CL1/Fractalkine Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CX3CL1/Fractalkine
Gln25-Gly100
Accession # Q6I9S9
Specificity
Detects human CX3CL1/Fractalkine in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse CX3CL1 and recombinant rat CX3CL1 is observed and less than 5% cross-reactivity with recombinant human (rh) MCP‑2 and rhMCP-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CX3CL1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.3-1.5 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF365 in the following applications:

Publications
Read Publications using
AF365 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody [Unconjugated]

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for human CX3CL1 has been mapped to chromosome 16q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CX3CL1/Fractalkine Antibody (AF365)(17)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 8 applications: ELISA Development, Flow, ICC, IHC - Paraffin embedded, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
ELISA Development
(1)
Flow
(1)
ICC
(1)
IHC - Paraffin embedded
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(4)
Neut
(2)
WB
(6)
All Applications
Filter By Species
Human
(16)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF365 Applications Species
Erreni M, Siddiqui I, Marelli G, Grizzi F, Bianchi P, Morone D, Marchesi F, Celesti G, Pesce S, Doni A, Rumio C, Roncalli M, Laghi L, Mantovani A, Allavena P The Fractalkine-Receptor Axis Improves Human Colorectal Cancer Prognosis by Limiting Tumor Metastatic Dissemination. J Immunol, 2016;196(2):902-14. 2016 [PMID: 26673138] (IHC - Paraffin embedded,, Human) IHC - Paraffin embedded Human
Kassianos A, Wang X, Sampangi S, Afrin S, Wilkinson R, Healy H Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells. Kidney Int, 2015;87(6):1153-63. 2015 [PMID: 25587706] (IHC Frozen,, Human) IHC Frozen Human
Brissot E, Bossard C, Malard F, Braudeau C, Chevallier P, Guillaume T, Delaunay J, Josien R, Gregoire M, Gaugler B, Mohty M Involvement of the CX3CL1 (fractalkine)/CX3CR1 pathway in the pathogenesis of acute graft-versus-host disease. J Leukoc Biol, 2015;97(2):227-35. 2015 [PMID: 25420917] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y, Sun Z Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy. Mol Med Rep, 2013;8(6):1817-23. 2013 [PMID: 24068281] (WB,, Human) WB Human
Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D Expression and regulation of chemokines in murine and human type 1 diabetes. Diabetes, 2012;61(2):436-46. 2012 [PMID: 22210319] (Flow,, Human) Flow Human
Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP Fractalkine is a novel human adipochemokine associated with type 2 diabetes. Diabetes, 2011;60(5):1512-8. 2011 [PMID: 21525510] (Neut,, Human) Neut Human
Tole S, Durkan AM, Huang YW, Liu GY, Leung A, Jones LL, Taylor JA, Robinson LA Thromboxane prostanoid receptor stimulation induces shedding of the transmembrane chemokine CX3CL1 yet enhances CX3CL1-dependent leukocyte adhesion. Am. J. Physiol., Cell Physiol., 2010;298(6):C1469-80. 2010 [PMID: 20237149] (WB,, Human) WB Human
Huang YW, Su P, Liu GY, Crow MR, Chaukos D, Yan H, Robinson LA Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases. J. Biol. Chem., 2009;284(43):29644-53. 2009 [PMID: 19723636] (ICC,, Human) ICC Human
Dorgham K, Ghadiri A, Hermand P, Rodero M, Poupel L, Iga M, Hartley O, Gorochov G, Combadiere C, Deterre P An engineered CX3CR1 antagonist endowed with anti-inflammatory activity. J. Leukoc. Biol., 2009;86(4):903-11. 2009 [PMID: 19571253] (ELISA Development,, Human) ELISA Development Human
Zanchi C, Zoja C, Morigi M, Valsecchi F, Liu XY, Rottoli D, Locatelli M, Buelli S, Pezzotta A, Mapelli P, Geelen J, Remuzzi G, Hawiger J Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin. J. Immunol., 2008;181(2):1460-9. 2008 [PMID: 18606701] (WB,, Human) WB Human
Show All 17 Publications.

Reviews for CX3CL1/Fractalkine Antibody (AF365) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF365:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22943205
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22943205

Details

Detection NotesSee PMID 22943205

Comments

CommentsPublished: See PMID 22943205Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Human 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23470165
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23470165

Details

Detection NotesSee PMID 23470165

Comments

CommentsPublished: See PMID 23470165Independent review from antibodyresource.com

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FAQs for CX3CL1/Fractalkine Antibody (AF365) (0)

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Secondary Antibodies

 

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
01/08/2015
Application: IF
Species: Human

 
Anonymous
01/08/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol CX3CL1
Entrez
Uniprot