GDF-11/GDF-8 Antibody (743835R) [Unconjugated]

Recombinant Human GDF-11/BMP-11 (Catalog # 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF-11/BMP-11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582R). The ND50 is typically 0.04-0.2 μg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay.

Recombinant Human GDF‑11/BMP‑11 protein was serially diluted 2-fold and captured by Mouse Anti-Human GDF‑11/BMP‑11 Monoclonal Antibody (Catalog # MAB19584) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GDF‑11/GDF‑8 Monoclonal Antibody (Catalog # MAB19582R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA, B/N
• Clone: 743835R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GDF-11/GDF-8 Antibody (743835R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human GDF-11/GDF-8
  Asn299-Ser407
  Accession # O95390
• Specificity: Detects human GDF-11/GDF-8 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Neutralization 0.04-0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-11/GDF-8 Antibody (743835R) [Unconjugated]

• GDF-11/GDF-8

Background

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide processed into a 109 aa mature protein. Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. Mature GDF-11 and GDF-8 share 90% amino acid sequence identity. GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (2). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (3). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (4, 5).

1. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
2. Oh, S.P. et al. (2002) Genes & Dev. 16:274.
3. Loffredo, F.S. et. al. (2013) Cell. 153.828.
4. Katsimpardi, L. et. al. (2014) Science. 344.630.
5. Sinha, M. et. al. (2014) Science. 344.649.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O95390()