Recombinant Human GDF-11/BMP-11 Animal-Free Protein

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SDS-Page: Recombinant Human GDF-11/BMP-11 Animal-Free Protein [NBP2-76279] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Human GDF-11/BMP-11 Animal-Free Protein [NBP2-76279] - GDF-11/BMP-11 alkaline phosphatase activity in ATDC-5 cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-76279
    • Availability
      Product Discontinued

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Recombinant Human GDF-11/BMP-11 Animal-Free Protein Summary

Description
For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
Specificity
GDF-11/BMP-11 is a dimer, 12.5/24.9 kDa (109/218 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
Alkaline phosphatase activity in ATDC5 cells, >1.0 x 10^4 units/mg (ED50 acceptance criteria are <100 ng/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GDF11
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Application Notes
Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile 10 mM HCl at 0.1 mg/mL

Alternate Names for Recombinant Human GDF-11/BMP-11 Animal-Free Protein

  • BMP11
  • BMP-11
  • BMP-11BMP11Bone morphogenetic protein 11
  • GDF11
  • GDF-11
  • growth differentiation factor 11
  • growth/differentiation factor 11

Background

BMP11 is encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GDF11