Recombinant Human GDF-11/BMP-11 Animal-Free Protein

SDS-Page: Recombinant Human GDF-11/BMP-11 Animal-Free Protein [NBP2-76279] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Human GDF-11/BMP-11 Animal-Free Protein [NBP2-76279] - GDF-11/BMP-11 alkaline phosphatase activity in ATDC-5 cells.

Product Discontinued

Recombinant Human GDF-11/BMP-11 Animal-Free Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: GDF-11/BMP-11 is a dimer, 12.5/24.9 kDa (109/218 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: Alkaline phosphatase activity in ATDC5 cells, >1.0 x 10^4 units/mg (ED50 acceptance criteria are <100 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GDF11
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 24.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile 10 mM HCl at 0.1 mg/mL

Alternate Names for Recombinant Human GDF-11/BMP-11 Animal-Free Protein

• BMP11
• BMP-11
• BMP-11BMP11Bone morphogenetic protein 11
• GDF11
• GDF-11
• growth differentiation factor 11
• growth/differentiation factor 11

Background

BMP11 is encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GDF11