ALK-7/Activin Receptor Type 1C Antibody (118311) [Unconjugated]

• Reactivity: Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: 118311
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ALK-7/Activin Receptor Type 1C Antibody (118311) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat ALK‑7
  Leu26-Arg108
  Accession # NP_620790
• Specificity: Detects rat ALK-7 in Western blots. In Western blots, no cross-reactivity with recombinant human Activin R1B is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACVR1C
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALK-7/Activin Receptor Type 1C Antibody (118311) [Unconjugated]

• activin A receptor, type IC
• Activin receptor type IC
• activin receptor type-1C
• Activin receptor-like kinase 7
• Activin RIC
• ACTR-IC
• ACVR1C
• ACVRLK7
• ALK7
• ALK-7
• ALK7ALK-7
• EC 2.7.11
• EC 2.7.11.30

Background

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. ALK-7 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. Rat ALK-7 expression is restricted to the adult central nervous system, suggesting a possible role of ALK-7 during postnatal maturation and maintenance of several neuronal subpopulations in the cerebellum.

1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
2. ten Dijke, P. et al. (1994) Science 264:101.
3. Ryden, M. et al. (1996) J. Biol. Chem. 271:30603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ALK-7/Activin Receptor Type 1C Antibody (MAB5771) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous WB Rat 02/25/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23830891
• Species: Rat

Blocking

• Blocking Details: See PMID 23830891

Details

• Detection Notes: See PMID 23830891

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23830891

Bioinformatics

• Gene Symbol: ACVR1C
• Uniprot:
  • NP_620790()