GCKR Antibody

Western Blot: GCKR Antibody [NBP2-16623] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: GCKR Antibody [NBP2-16623] - Human normal colon, using GCKR antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GCKR Antibody Summary

• Description: Novus Biologicals Rabbit GCKR Antibody (NBP2-16623) is a polyclonal antibody validated for use in IHC and WB. Anti-GCKR Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GCKR. The exact sequence is proprietary.
• Predicted Species: Rat (92%), Canine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCKR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GCKR Antibody

• FGQTL5
• GKRP
• glucokinase (hexokinase 4) regulator
• glucokinase (hexokinase 4) regulatory protein
• Glucokinase regulator
• glucokinase regulatory protein

Background

This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GCKR Antibody (NBP2-16623)(2)

Publications using NBP2-16623

• Alshamrani AA, Ibrahim MMH, Briski KP Effects of short-term food deprivation on catecholamine and metabolic-sensory biomarker gene expression in hindbrain A2 noradrenergic neurons projecting to the forebrain rostral preoptic area: Impact of negative versus positive estradiol feedback IBRO neuroscience reports 2022-12-01 [PMID: 35711244] (IF/IHC, Rat)
• Ali MH, Alshamrani AA, Briski KP Hindbrain lactate regulation of hypoglycemia-associated patterns of catecholamine and metabolic-sensory biomarker gene expression in A2 noradrenergic neurons innervating the male versus female ventromedial hypothalamic nucleus Journal of chemical neuroanatomy 2022-04-25 [PMID: 35483611] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: GCKR
• Uniprot:
  • Q14397(Human)