Melatonin Receptor 1B Antibody

Images

 
Western Blot: Melatonin Receptor 1B Antibody [NLS932] - Analysis of 1. HepG2 and 2. A431.
Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Melatonin receptor type 1 B (MTR1B) in rat tissues fixed in paraformaldehyde and embedded in paraffin. A,B-brain, C-prostate. Image from verified ...read more
Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Analysis of Human Brain. 8 ug/ ml.
Immunohistochemistry: Melatonin Receptor 1B Antibody [NLS932] - Analysis of Human Brain using Fast Red with hematoxylin counterstain.
Immunohistochemistry: Melatonin Receptor 1B Antibody [NLS932] - Staining of Mouse Brain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Melatonin Receptor 1B Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human Melatonin Receptor 1B protein (within residues 200-300) [Swiss-Prot P49286].
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTNR1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This Melatonin Receptor 1B antibody is useful for Immunohistochemistry with formalin fixed paraffin embedded tissues and Western blot. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24365461)
Control Peptide
Melatonin Receptor 1B Peptide (NLS932PEP)
Reviewed Applications
Read 1 Review rated 4
using
NLS932 in the following applications:

Publications
Read Publications using
NLS932 in the following applications:

Reactivity Notes

Human, mouse and rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Melatonin Receptor 1B Antibody

  • FGQTL2
  • Mel 1b melatonin receptor
  • Mel1b receptor
  • MEL-1B-R
  • melatonin receptor 1B
  • melatonin receptor MEL1B
  • melatonin receptor type 1B
  • MT2

Background

Melatonin receptor type 1B (MTNR1B) is a member of the G-protein coupled receptor 1 family. This family is responsible for translating external chemical signals across the cell membrane. MTNR1B is one of two high affinity receptors for melatonin, a hormone secreted by the pineal gland. Pertussis toxin sensitive G proteins, that inhibit adenylate cyclase activity, control the MTNR1B receptor. MTNR1B is expressed in the retina, and to a lesser extent the brain and hippocampus. MTNR1B is thought to regulate reproduction, the circadian cycle, and neurological melatonin levels; as well as participate in light dependent function in the retina. Post translational modifications that may affect MTNR1B are disulfide bonds and glycosylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melatonin Receptor 1B Antibody (NLS932)(2)

Review for Melatonin Receptor 1B Antibody (NLS932) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NLS932:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Melatonin Receptor 1B NLS932
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reviewed by:
Anonymous
IHC-P Rat 09/03/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRat ventral prostate and brain.
SpeciesRat
LotA-1

Blocking

Blocking DetailsPeroxidase Blocking H202 9% in water solution, 15 min; Non specific protein in BSA 3%, 1 hour.

Primary Anitbody

Dilution Ratio1:80, in BSA 1%, 4oC, overnight

Secondary Antibody

Secondary Descriptiongoat anti-rabbit
Secondary Manufacturer Cat#sc2018, rabbit, Santa Cruz Biotecnology
Secondary Concentration1:200

Details

Detection NotesNegative control: without primary antibody. Positive control: brain
Fixation DetailsFixation: Paraformoldehyde; Permeabilization: Ethanol and Xilol, Antigen Retrieval in citrate buffer 0,1M pH 6, 92oC, 15 minutes
Wash DescriptionPhosphate buffer PBS pH 7, 3X during 5 minutes

Product General Protocols

View specific protocols for Melatonin Receptor 1B Antibody (NLS932): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Melatonin Receptor 1B Antibody (NLS932). (Showing 1 - 2 of 2 FAQs).

  1. I saw that you have the antibodies for the both receptors of melatonin but not listed with FACS application. I need more information about what is better for my assay.
    • At this time, none of our melatonin receptor antibodies have been tested and validated in Flow. Should you choose to purchase and test one of them, you would qualify for the Innovator's Rewards program. You should take a look at each individual antibody, the clonality, conjugates, immunogen region and all relevant data in order to decide which product would be most suitable for your needs.
  2. Would you let me know the tissue for positive control of NLS932?
    • This antibody was tested in both mouse brain and human brain. These are our recommended positive controls. According to UniProt, this protein is also present in retina and hippocampus (UniProt P49286) so these may also be suitable.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
09/03/2014
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol MTNR1B
Entrez
Uniprot