GBX2 Antibody

Western Blot: GBX2 Antibody [NBP2-43617] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.

Western Blot: GBX2 Antibody [NBP2-43617] - Analysis of IMR32 whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted by 1:1000.

Western Blot: GBX2 Antibody [NBP2-43617] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.

Western Blot: GBX2 Antibody [NBP2-43617] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.

Western Blot: GBX2 Antibody [NBP2-43617] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (NBP2-43617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: GBX2 Antibody [NBP2-43617] - GBX2 antibody detects GBX2 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (NBP2-43617) diluted by 1:1000.

GBX2 antibody detects GBX2 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GBX2 protein stained by GBX2 antibody (NBP2-43617) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GBX2 Antibody Summary

• Description: Novus Biologicals Rabbit GBX2 Antibody (NBP2-43617) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GBX2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GBX2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GBX2 Antibody

• Gastrulation and brain-specific homeobox protein 2
• gastrulation brain homeo box 2
• gastrulation brain homeobox 2
• GBX2
• homeobox protein GBX-2

Background

GBX2 (Gastrulation brain homeobox 2) is a homeobox protein which is involved in enbryonic cell pluripotency and differentiation. GBX2 is expressed in all three embryonic germ layers of the late gastrula/early neurula. Gbx2 is overexpressed in a panel of human prostatic cancer cell lines (TSU-pr1, PC3, DU145, LNCaP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GBX2