GATA-3 Antibody - BSA Free

Western Blot: GATA-3 Antibody [NBP1-77115] - Analysis in K562 cell lysate with antibody at 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunofluorescence of GATA-3 in Rat Brain tissue with GATA-3 antibody at 20 u/mL.

Immunohistochemistry: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunohistochemistry of GATA-3 in mouse brain tissue with GATA-3 antibody at 2.5 ug/ml.

Immunohistochemistry: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunohistochemistry of GATA-3 in rat brain tissue with GATA-3 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunofluorescence of GATA-3 in mouse brain tissue with GATA-3 Antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GATA-3 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3. The immunogen is located within amino acids 340 - 390 of GATA3. Amino Acid Squence: KEGIQTRNRKMSSKSKK
• Predicted Species: Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATA3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  GATA-3 Peptide (NBP1-77115PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for GATA-3 Antibody - BSA Free

• 131320
• GATA binding protein 3
• GATA3
• GATA-3
• GATA-binding factor 3
• GATA-binding protein 3
• HDRMGC5199
• MGC2346
• MGC5445
• trans-acting T-cell-specific transcription factor GATA-3

Background

GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-3 Antibody (NBP1-77115)(1)

Publication using NBP1-77115

• Han L, Huang Y, Fu Q et al. Tracking the response to Pseudomonas aeruginosa infection in ozone-induced chronic obstructive pulmonary disease mouse models Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-04-19 [PMID: 35453008] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: GATA3
• Uniprot:
  • NP_001002295(Human)