GATA-3 Antibodies
We offer GATA-3 Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each GATA-3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GATA-3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the GATA-3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GATA-3 polyclonal antibodies and browse our GATA-3 monoclonal antibody catalog.
Alternate Names for GATA-3 Antibodies
anti-GATA-3 antibody, anti-GATA3 antibody, anti-131320 antibody, anti-GATA binding protein 3 antibody, anti-GATA-binding factor 3 antibody, anti-GATA-binding protein 3 antibody, anti-HDRMGC5199 antibody, anti-MGC2346 antibody, anti-MGC5445 antibody, anti-trans-acting T-cell-specific transcription factor GATA-3 antibody
54 Results for "gata-3" in Primary Antibodies
![Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis (35 ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody at 1:200.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Western-Blot-NBP2-45810-img0013.jpg "Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis (35 ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody at 1:200.")
![Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Mouse lung tissue section stained with GATA-3 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GATA-3-Antibody-OTI8H4-Immunohistochemistry-Paraffin-NBP2-45810-img0014.jpg)
![Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Flow cytometric Analysis of penetrated MCF-7 cells, compared to a nonspecific negative control antibody, (Blue). The right is HUVEC cells as negtive control (1:100).](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Flow-Cytometry-NBP2-45810-img0011.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0001.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0002.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human colon tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0003.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human Kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0004.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0005.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human endometrium tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0006.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0007.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0008.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human bladder tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0009.jpg)
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human lymphoma tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0010.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human cell line TD47D.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Western-Blot-NBP2-88917-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of MCF7 cells using the Anti-GATA3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunocytochemistry-Immunofluorescence-NBP2-88917-img0001.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0002.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human kidney shows strong nuclear positivity in a small subset of cells in glomeruli.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0003.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0004.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0005.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0006.jpg)
![Western Blot: GATA-3 Antibody [NBP1-77115] - Analysis in K562 cell lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/GATA-3-Antibody-Western-Blot-NBP1-77115-img0010.jpg "Western Blot: GATA-3 Antibody [NBP1-77115] - Analysis in K562 cell lysate with antibody at 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunofluorescence of GATA-3 in Rat Brain tissue with GATA-3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-2711202420212392.jpg)
![Immunohistochemistry: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunohistochemistry of GATA-3 in mouse brain tissue with GATA-3 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-2711202419463470.gif)
![Immunohistochemistry: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunohistochemistry of GATA-3 in rat brain tissue with GATA-3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-2711202419172230.jpg)
![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunofluorescence of GATA-3 in mouse brain tissue with GATA-3 Antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-271120242042572.gif)
![Western Blot: GATA-3 Antibody (BLR121H) [NBP3-14705] - Whole cell lysate (50 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-BLR121H-Western-Blot-NBP3-14705-img0003.jpg "Western Blot: GATA-3 Antibody (BLR121H) [NBP3-14705] - Whole cell lysate (50 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (BLR121H) [NBP3-14705] - Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/GATA-3-Antibody-BLR121H-Immunocytochemistry-Immunofluorescence-NBP3-14705-img0005.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (BLR121H) [NBP3-14705] - Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/GATA-3-Antibody-BLR121H-Immunohistochemistry-Paraffin-NBP3-14705-img0004.jpg)
![Western Blot: GATA-3 Antibody (BLR121H) [NBP3-14705] - Whole cell lysate (10 ug) from HEK293T, Hep-G2, Jurkat, RKO, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds.](http://images.novusbio.com/fullsize/GATA-3-Antibody-BLR121H-Western-Blot-NBP3-14705-img0002.jpg)
![Immunoprecipitation: GATA-3 Antibody [NBP3-14705] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MCF-7 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiGATA3 recombinant monoclonal antibody used for IP at 6 ul per reaction. GATA3was also immunoprecipitated by rabbit anti-GATA3antibody For blotting immunoprecipitatedGATA3, A700-121 was used at 1:1000.Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/GATA-3-Antibody-Immunoprecipitation-NBP3-14705-img0001.jpg)
![Western Blot: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Western blot analysis of GATA-3 on different lysates with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/37/25 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32375) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-27820241631676.jpg "Western Blot: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Western blot analysis of GATA-3 on different lysates with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/37/25 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32375) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32375) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-298202414161838.jpg)
![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Immunocytochemistry analysis of SH-SY5Y cells labeling GATA-3 with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-29202410234242.jpg)
![Flow Cytometry: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Flow cytometric analysis of SH-SY5Y cells labeling GATA-3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32375, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-29202416372173.jpg)
![Western Blot: GATA-3 Antibody (7B5) [NBP1-47397] - Analysis using GATA3 mouse mAb against GATA3(AA: full length)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/GATA-3-Antibody-7B5-Western-Blot-NBP1-47397-img0005.jpg "Western Blot: GATA-3 Antibody (7B5) [NBP1-47397] - Analysis using GATA3 mouse mAb against GATA3(AA: full length)-hIgGFc transfected HEK293 cell lysate.")
![Immunohistochemistry-Paraffin: GATA-3 Antibody (7B5) [NBP1-47397] - Analysis of human Small Intestine, muscularis propria tissues using anti-GATA3 mouse mAb](http://images.novusbio.com/fullsize/GATA-3-Antibody-7B5-Immunohistochemistry-Paraffin-NBP1-47397-img0004.jpg)
![Western Blot: GATA-3 Antibody (PD00-41) [NBP3-32374] - Western blot analysis of GATA-3 on different lysates with Mouse anti-GATA-3 antibody (NBP3-32374) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 48/35 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32374) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32374_mouse-gata-3-mab-pd00-41-278202415212428.jpg "Western Blot: GATA-3 Antibody (PD00-41) [NBP3-32374] - Western blot analysis of GATA-3 on different lysates with Mouse anti-GATA-3 antibody (NBP3-32374) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 48/35 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32374) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GATA-3 Antibody (PD00-41) [NBP3-32374] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-GATA-3 antibody (NBP3-32374) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32374) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32374_mouse-gata-3-mab-pd00-41-298202414262623.jpg)
![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (PD00-41) [NBP3-32374] - Immunocytochemistry analysis of SH-SY5Y cells labeling GATA-3 with Mouse anti-GATA-3 antibody (NBP3-32374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GATA-3 antibody (NBP3-32374) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32374_mouse-gata-3-mab-pd00-41-2920241023423.jpg)