GATA-3 Antibody (CL8660) - Azide and BSA Free

Analysis in human skin and pancreas tissues using NBP3-44195 antibody. Corresponding GATA-3 RNA-seq data are presented for the same tissues.

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Staining of human kidney shows strong nuclear positivity in a small subset of cells in glomeruli.

Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.

Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Analysis in human cell line TD47D.

Staining of MCF7 cells using the Anti-GATA-3 monoclonal antibody) .

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, ChIP
• Clone: CL8660
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GATA-3 Antibody (CL8660) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GATA-3 Antibody (CL8660) - Azide and BSA Free (NBP2-88917) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P23771, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for GATA-3 Antibody (CL8660) - Azide and BSA Free

• 131320
• GATA binding protein 3
• GATA3
• GATA-3
• GATA-binding factor 3
• GATA-binding protein 3
• HDRMGC5199
• MGC2346
• MGC5445
• trans-acting T-cell-specific transcription factor GATA-3

Background

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA3