GATA-3 Antibody (CL8660) - BSA Free

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.

Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human cell line TD47D.

Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of MCF7 cells using the Anti-GATA3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human kidney shows strong nuclear positivity in a small subset of cells in glomeruli.

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL8660
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

GATA-3 Antibody (CL8660) - BSA Free Summary

• Immunogen: This antibody was developed using a synthetic peptide derived from P23771, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, containing 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for GATA-3 Antibody (CL8660) - BSA Free

• 131320
• GATA binding protein 3
• GATA3
• GATA-3
• GATA-binding factor 3
• GATA-binding protein 3
• HDRMGC5199
• MGC2346
• MGC5445
• trans-acting T-cell-specific transcription factor GATA-3

Background

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA3