GATA-3 Antibody (BLR121H) - BSA Free

Western Blot: GATA-3 Antibody (BLR121H) [NBP3-14705] - Whole cell lysate (50 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.

Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (BLR121H) [NBP3-14705] - Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: GATA-3 Antibody (BLR121H) [NBP3-14705] - Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Western Blot: GATA-3 Antibody (BLR121H) [NBP3-14705] - Whole cell lysate (10 ug) from HEK293T, Hep-G2, Jurkat, RKO, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody [BLR121H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds.

Immunoprecipitation: GATA-3 Antibody [NBP3-14705] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MCF-7 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiGATA3 recombinant monoclonal antibody used for IP at 6 ul per reaction. GATA3was also immunoprecipitated by rabbit anti-GATA3antibody For blotting immunoprecipitatedGATA3, A700-121 was used at 1:1000.Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BLR121H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GATA-3 Antibody (BLR121H) - BSA Free Summary

• Description: Novus Biologicals Rabbit GATA-3 Antibody (BLR121H) - BSA Free (NBP3-14705) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: between 150 and 200
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GATA3
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100- 1:500
  • Immunohistochemistry 1:100- 1:500
  • Immunohistochemistry-Paraffin 1:100- 1:500
  • Immunoprecipitation 6 ul/1 mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for GATA-3 Antibody (BLR121H) - BSA Free

• 131320
• GATA binding protein 3
• GATA3
• GATA-3
• GATA-binding factor 3
• GATA-binding protein 3
• HDRMGC5199
• MGC2346
• MGC5445
• trans-acting T-cell-specific transcription factor GATA-3

Background

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA3