GATA-2 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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Catalog# & Conjugate Size Price

GATA-2 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769
Specificity
Detects human GATA-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-5, and rhGATA-6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-2 Antibody [Biotin]

  • endothelial transcription factor GATA-2
  • FLJ45948
  • GATA binding protein 2
  • GATA2
  • GATA-2
  • GATA-binding protein 2NFE1B
  • MGC2306

Background

GATA proteins are transcriptional coactivators with two GATA-type zinc fingers. GATAs bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1, -2, -3 are selectively expressed in hematopoietic and endothelial cells, while GATA-4, -5, and -6 are expressed in developing heart and gut-derived tissues. GATA-1 and -3 exist in multiple alternatively spliced isoforms.

GATA-2 is expressed in hematopoietic progenitors and stem cells. GATA-2 expression is associated with cell proliferation. GATA-2 is 480 amino acids in length and contains 2 Zn2+ finger domains responsible for DNA binding (aa 295 - 319 and 349 - 373). Over aa 15 - 279 human GATA-2 share 96% identity with mouse GATA-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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Bioinformatics

Gene Symbol GATA2
Entrez
Uniprot