Gas6 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Gas6 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Gas6 (R&D Systems, Catalog # 986-GS)
  Asp115-Pro673
  Accession # CAA42507
• Specificity: Detects mouse Gas6 in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant human Gas6 is observed and less than 1% cross-reactivity with recombinant mouse Gas1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GAS6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Gas6 Antibody (Catalog # AF986)
  ELISA Detection: Mouse Gas6 Biotinylated Antibody (Catalog # BAF986)
  Standard: Recombinant Mouse Gas6 (Catalog # 986-GS)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gas6 Antibody [Biotin]

• AXLLG
• AXLLGAXL stimulatory factor
• AXSFAXL receptor tyrosine kinase ligand
• DKFZp666G247
• FLJ34709
• Gas6
• GAS-6
• growth arrest-specific 6
• growth arrest-specific protein 6

Background

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium.

Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

1. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
2. Goruppi, S. et al. (1996) Oncogene 12:471.
3. Crosier, K and P. Crosier (1997) Pathology 29:131.
4. Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
5. Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gas6 Antibody (BAF986)(6)

Publications using BAF986

• Aydin, N;Ouliass, B;Ferland, G;Hafizi, S; Modification of Gas6 Protein in the Brain by a Functional Endogenous Tissue Vitamin K Cycle Cells 2024-05-18 [PMID: 38786095] (ELISA Detection, Mouse)
• Naoya Fujino, Oliver J. Brand, David J. Morgan, Toshifumi Fujimori, Aleksander M. Grabiec, Christopher P. Jagger et al. Sensing of apoptotic cells through Axl causes lung basal cell proliferation in inflammatory diseases Journal of Experimental Medicine 2019-09-02 [PMID: 31289116]
• Mihalis S Kariolis Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies J. Clin. Invest, 2016-11-28;0(0):. 2016-11-28 [PMID: 27893463] (ELISA Development (Detection), Mouse)
• Fujimori T, Grabiec A, Kaur M, Bell T, Fujino N, Cook P, Svedberg F, MacDonald A, Maciewicz R, Singh D, Hussell T The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung. Mucosal Immunol, 2015-01-21;8(5):1021-30. 2015-01-21 [PMID: 25603826] (Flow Cytometry, Mouse)
• Mihalis S. Kariolis, Yu Rebecca Miao, Douglas S. Jones, Shiven Kapur, Irimpan I. Mathews, Amato J. Giaccia et al. An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis Nature Chemical Biology 2014-11-01 [PMID: 25242553]
• Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P Role of Gas6 in erythropoiesis and anemia in mice. J. Clin. Invest., 2008-02-01;118(2):583-96. 2008-02-01 [PMID: 18188450] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: GAS6
• Uniprot:
  • CAA42507()