Dtk/TYRO3 Antibody (96201) [Unconjugated]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Tyro3/Dtk knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human Tyro3/Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Tyro3/Dtk knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, IHC, ELISA(Cap), KO
• Clone: 96201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Dtk/TYRO3 Antibody (96201) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tyro3/Dtk
  Ala41-Ser428 (predicted)
  Accession # Q06418
• Specificity: Detects human Tyro3/Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interferance with recombinant mouse Tyro3/Dtk, recombinant human (rh) Axl, or rhMer is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TYRO3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated 0.25 ug/10^6 cells
• Application Notes: ELISA Detection: Human Dtk Biotinylated Antibody (Catalog number BAF859)
  Standard: Recombinant Human Dtk Fc Chimera (Catalog number 859-DK)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dtk/TYRO3 Antibody (96201) [Unconjugated]

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Dtk (also called Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dtk/TYRO3 Antibody (MAB859)(8)

Publications using MAB859

• J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK Arthritis Research & Therapy, 2020;22(1):221. 2020 [PMID: 32958023] (Bioassay, Human)
• SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation J. Immunol., 2017;0(0):. 2017 [PMID: 28916522] (Western Blot, Human)
• KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication Sci Rep, 2017;7(0):41389. 2017 [PMID: 28128342] (ICC, Human)
• Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. 2012 [PMID: 23071254] (Flow Cytometry, Human)
• Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. J. Virol., 2012;86(4):2067-78. 2012 [PMID: 22156524] (Flow Cytometry, Human)
• Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Thromb. Haemost., 2011;107(1):140-9. 2011 [PMID: 22072012] (ICC, Human)
• Zhu D, Wang Y, Singh I Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010;115(23):4963-72. 2010 [PMID: 20348395] (Western Blot, Human)
• Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions. Am. J. Pathol., 2009;175(1):283-93. 2009 [PMID: 19541935] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: TYRO3
• Uniprot:
  • Q06418()