Dtk/TYRO3 Antibody (96201) [Unconjugated]

Images

 
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by ...read more
Tyro3/Dtk knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human Tyro3/Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, ELISA(Cap), KO
Clone
96201
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Dtk/TYRO3 Antibody (96201) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tyro3/Dtk
Ala41-Ser428 (predicted)
Accession # Q06418
Specificity
Detects human Tyro3/Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interferance with recombinant mouse Tyro3/Dtk, recombinant human (rh) Axl, or rhMer is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TYRO3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated 0.25 ug/10^6 cells
Application Notes
ELISA Detection: Human Dtk Biotinylated Antibody (Catalog number BAF859)
Standard: Recombinant Human Dtk Fc Chimera (Catalog number 859-DK)
Publications
Read Publications using
MAB859 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dtk/TYRO3 Antibody (96201) [Unconjugated]

  • Brt
  • BYK
  • Dtk
  • EC 2.7.10
  • EC 2.7.10.1
  • FLJ16467
  • Rse
  • Sky
  • Tif
  • TYRO3 protein tyrosine kinase
  • Tyro3
  • Tyrosine-protein kinase byk
  • Tyrosine-protein kinase DTK
  • tyrosine-protein kinase receptor TYRO3
  • Tyrosine-protein kinase RSE
  • Tyrosine-protein kinase SKY

Background

Dtk (also called Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dtk/TYRO3 Antibody (MAB859)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Bioassay, Flow Cytometry, ICC, IHC-Fr, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(2)
ICC
(2)
IHC-Fr
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB859 Applications Species
J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK Arthritis Research & Therapy, 2020;22(1):221. 2020 [PMID: 32958023] (Bioassay, Human) Bioassay Human
SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation J. Immunol., 2017;0(0):. 2017 [PMID: 28916522] (Western Blot, Human) Western Blot Human
KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication Sci Rep, 2017;7(0):41389. 2017 [PMID: 28128342] (ICC, Human) ICC Human
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. 2012 [PMID: 23071254] (Flow Cytometry, Human) Flow Cytometry Human
Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. J. Virol., 2012;86(4):2067-78. 2012 [PMID: 22156524] (Flow Cytometry, Human) Flow Cytometry Human
Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Thromb. Haemost., 2011;107(1):140-9. 2011 [PMID: 22072012] (ICC, Human) ICC Human
Zhu D, Wang Y, Singh I Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010;115(23):4963-72. 2010 [PMID: 20348395] (Western Blot, Human) Western Blot Human
Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions. Am. J. Pathol., 2009;175(1):283-93. 2009 [PMID: 19541935] (IHC-Fr, Human) IHC-Fr Human

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Bioinformatics

Gene Symbol TYRO3
Uniprot