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HAF109). Specific bands were detected for Gas6 at approximately 64 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
986-GS) was serially diluted and captured by Goat Anti-Mouse Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF986) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF986) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
986-GS) was serially diluted and captured by Goat Anti-Mouse Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF986) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF986) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 25-150 ng/mL." class="big_lightbox" target="_blank">
Growth arrest specific proteins, including Gas1 and Gas6, are activated in quiescent cells. Gas1-induced growth arrest is mediated by p53, and Gas1 appears to be able to suppress tumor cell growth. Gas6, a ligand for the tyrosine kinase receptor Axl, was initially identified as a member of the vitamin K-dependent protein family and exhibits a high degree of amino acid sequence homology to protein S, a negative co-regulator in the coagulation pathway.