GABARAPL1 Antibody

Western Blot: GABARAPL1 Antibody [NBP3-13125] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with GABARAPL1 antibody (NBP3-13125) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GABARAPL1 Antibody [NBP3-13125] - GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABARAPL1 protein stained by GABARAPL1 antibody (NBP3-13125) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.

Western Blot: GABARAPL1 Antibody [NBP3-13125] - Various whole cell extracts (60 ug) and tissue extracts (15 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL1 antibody (NBP3-13125) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Gp, RM, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GABARAPL1 Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAPL1. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Bovine (100%), Guinea Pig (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABARAPL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GABARAPL1 Antibody

• APG8L
• APG8-LIKE
• ATG8
• ATG8B
• ATG8L
• Early estrogen-regulated protein
• GABA(A) receptor-associated protein like 1
• GABARAPL1
• gamma-aminobutyric acid receptor-associated protein-like 1
• GEC1
• GEC-1
• GEC1GABA(A) receptor-associated protein-like 1
• Glandular epithelial cell protein 1

Background

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs)function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast. Starved yeast cells take up their own cytoplasm into vacuoles through autophagic bodies. Autophagic bodies form a double-membraned structure called the autophagosome,which subsequently fuses with the vacuole/lysosome. This process similar in mammals. Two sets of genes, APG and AUT, have been identified with this process, and are responsible for two ubiquitin-like systems Apg12 and Apg8, respectively. Apg12 is synthesized in its mature form and seems to have one target, Apg5. Almost all Apg12 molecules are conjugated with Apg5. Aut2/Apg4 processes the Apg8/Aut7 system at its carboxy-terminal region. Apg8 exists in two forms, one is membrane bound through a phospholipid. Lipidation/ activation of Apg8 is mediated by Apg7 and transferred to Apg3 and finally forms a conjugate with phosphatidyl-ethanolamine (PE). Apg4 cleaves Apg8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABARAPL1