GABARAP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GABARAP Antibody [NBP1-71771] - analysis of GABARAP in Raw 264.7 cells using anti-GABARAP antibody (red). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: GABARAP Antibody [NBP1-71771] - Analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: GABARAP Antibody [NBP1-71771] - GABARAP antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.11 mg/ml

GABARAP Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of the human GABARAP protein (within residues 50-117). [Swiss-Prot O95166]
Localization
Endomembrane system. Cytoplasm. Golgi apparatus membrane. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABARAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This GABARAP antibody is useful for ICC/IF and IHC-P. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse. Predicted to react with rat based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.11 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GABARAP Antibody

  • Apg8p1
  • ATG8A
  • FLC3B
  • FLJ25768
  • GABA(A) receptor-associated proteinMGC120154
  • GABARAP
  • gamma-aminobutyric acid receptor-associated protein
  • MM46
  • MM46MGC120155

Background

In several neurological diseases, GABARAP expression is related to autophagic functions. In Lewy body disease and Parkinson's disease, LC3 and GABARAP are often co-localized. Autophagy is regulated both at the transcriptional and post-transcriptional level, partially by the activation of GABARAP and other core autophagy machinery proteins. Although autophagy is usually considered to be important in cellular maintenance, and breakdown of proteins and organelles in cell death, it is becoming more and more clear that it plays an important role in the developmental process, including embryogenesis. This embryological effect seems to be partly regulated by GABARAP expression and activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GABARAP Antibody (NBP1-71771) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71771AF405
Alexa Fluor 488 Labeled NBP1-71771AF488
Alexa Fluor 647 Labeled NBP1-71771AF647
Alexa Fluor 700 Labeled NBP1-71771AF700
Biotin Labeled NBP1-71771B
DyLight 405 Labeled NBP1-71771V
DyLight 488 Labeled NBP1-71771G
DyLight 550 Labeled NBP1-71771R
DyLight 650 Labeled NBP1-71771C
FITC Labeled NBP1-71771F

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Bioinformatics Tool for GABARAP Antibody (NBP1-71771)

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Diseases for GABARAP Antibody (NBP1-71771)

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Blogs on GABARAP.

Breakdown: Interpreting LC3 Antibody WB Results
In rodents, MAPLC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form of LC3II, following conversion fr...  Read full blog post.

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Bioinformatics

Gene Symbol GABARAP
Entrez
Uniprot