GABA-AR alpha 6 Antibody

Western Blot: GABA-A R alpha 6 Antibody [NB300-150]

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GABA-AR alpha 6 Antibody Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the alpha 6 subunit conjugated to KLH. Accession # P30191
• Specificity: Specific for endogenous levels of the ~57 kDa alpha 6-subunit of the GABAA receptor.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRA6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:1000
• Application Notes: In Western blot a band at approximately 57 kDa is seen. Use in Immunohistochemistry reported in scientific literature (PMID 18509076)
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Cerebral Cortex Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for GABA-AR alpha 6 Antibody

• GABA A R alpha 6
• GABA subunit A receptor alpha 6
• GABA(A) receptor subunit alpha-6
• GABAAR alpha 6
• GABAARa6
• GABRA6
• gamma-aminobutyric acid (GABA) A receptor, alpha 6
• gamma-aminobutyric acid receptor subunit alpha-6
• MGC116903
• MGC116904

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. The alpha 6 subunit is expressed in cerebellar granule cells. Though it is concentrated at synapses, it is found on extrasynaptic membranes, as well.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 6 Antibody (NB300-150)(1)

Publication using NB300-150

• Krook-Magnuson EI, Li P, Paluszkiewicz SM et al. Tonically Active Inhibition Selectively Controls Feedforward Circuits in Mouse Barrel Cortex. J Neurophysiol;100(2):932-944. 2008-01-01 [PMID: 18509076] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: GABRA6
• Entrez:
  • 2559(Human)
  • 14399(Mouse)
  • 29708(Rat)
• Uniprot:
  • P30191(Rat)