GABA-AR alpha 1 Antibody

Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm
• Applications: WB, ICC/IF, IHC, IHC-Fr, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.15 mg/ml

GABA-AR alpha 1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P62813
• Localization: Membrane; multi pass membrane protein.
• Specificity: Specific for the ~51k alpha1-subunit of the GABAA receptor in Western blots of rat brain. Labeling is absent in alpha1-subunit knockout animals.
• Predicted Species: Canine (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22927941). Use in immunoprecipitation reported in scientific literature (PMID: 17404840).
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22927941)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Concentration: 0.15 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for GABA-AR alpha 1 Antibody

• ECA4
• EJM
• EJM5GABA(A) receptor subunit alpha-1
• GABA A R alpha 1
• GABAAR alpha 1
• GABAARa1
• GABRA1
• gamma-aminobutyric acid (GABA) A receptor, alpha 1
• gamma-aminobutyric acid receptor subunit alpha-1

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 1 Antibody (NB300-191)(10)

Publications using NB300-191

• Popoola DO, Nizhnikov ME, Cameron NM. Strain-specific programming of prenatal ethanol exposure across generations. Alcohol: An International Biomedical Journal 2017-01-03 [PMID: 28433421] (WB, Rat)
• Carlson SL, O'Buckley TK, Thomas R et al. Altered GABAA Receptor Expression and Seizure Threshold Following Acute Ethanol Challenge in Mice Lacking the RIIbeta Subunit of PKA. Neurochem Res. 2013-10-09 [PMID: 24104609] (WB, Mouse)
• Mendu SK, Bhandage A, Jin Z, Birnir B. Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes PLoS One 2012-01-01 [PMID: 22927941] (ICC/IF, Human, Mouse, Rat)
• Lin TY, Lu CW, Wang CC et al. Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals Toxicol Appl Pharmacol 2012-07-01 [PMID: 22759588] (WB, Rat)
• Kumar S, Ren Q, Beckley JH, O'Buckley TK, Gigante ED, Santerre JL, Werner DF, Morrow AL. Ethanol Activation of Protein Kinase A Regulates GABA(A) Receptor Subunit Expression in the Cerebral Cortex and Contributes to Ethanol-Induced Hypnosis. Front Neurosci;6:44. 2012-01-01 [PMID: 22509146] (WB, Rat)
• Suryanarayanan A, Liang J, Meyer EM et al. Subunit Compensation and Plasticity of Synaptic GABA(A) Receptors Induced by Ethanol in ?4 Subunit Knockout Mice. Front Neurosci;5:110. 2011-01-01 [PMID: 21977012] (WB, Mouse)
• Shen Y, Lindemeyer AK, Spigelman I et al. Plasticity of GABAA receptors after ethanol pre-exposure in cultured hippocampal neurons Mol Pharmacol 2011-03-01 [PMID: 21163967] (WB, Mouse)
• Kumar S, Suryanarayanan A, Boyd KN et al. Ethanol reduces GABAA alpha1 subunit receptor surface expression by a protein kinase Cgamma-dependent mechanism in cultured cerebral cortical neurons. Mol Pharmacol;77(5):793-803. 2010-05-01 [PMID: 20159950] (ICC/IF, Rat)
• Marutha Ravindran CR, Mehta AK, Ticku MK. Effect of chronic administration of ethanol on the regulation of tyrosine kinase phosphorylation of the GABAA receptor subunits in the rat brain. Neurochem Res 2007-07-01 [PMID: 17404840] (IP, Rat)
• Mehta AK, Ticku MK. Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: lack of modulation by chronic ethanol administration. Brain Res Mol Brain Res;67(1):194-9. 1999-04-06 [PMID: 10101248] (IP, Rat)

Review for GABA-AR alpha 1 Antibody (NB300-191) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 03/21/2017

Summary

• Application: Western Blot
• Sample Tested: brain and spinal cord,brain
• Species: Rat

FAQs for GABA-AR alpha 1 Antibody (NB300-191). (Showing 1 - 1 of 1 FAQs).

1. Are there neutralizing peptides for this antibody?
   • There is no neutralizing peptide available for this antibody.

Bioinformatics

• Gene Symbol: GABRA1
• Entrez:
  • 780973(Bovine)
  • 2554(Human)
  • 14394(Mouse)
  • 29705(Rat)
• Uniprot:
  • P62813(Rat)