GABA-AR alpha 1 Antibody

Images

 
Genetic Strategies: Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

GABA-AR alpha 1 Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P62813
Localization
Membrane; multi pass membrane protein.
Specificity
Specific for endogenous levels of the ~51 kDa alpha1-subunit of the GABAA receptor. Immunolabeling is absent in alpha1-subunit knockout animals.
Predicted Species
Canine (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABRA1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22927941). Use in immunoprecipitation reported in scientific literature (PMID: 17404840).
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-191 in the following applications:

Publications
Read Publications using
NB300-191 in the following applications:

  • 2 publications
  • IP
    2 publications
  • WB
    7 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22927941)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for GABA-AR alpha 1 Antibody

  • ECA4
  • EJM
  • EJM5GABA(A) receptor subunit alpha-1
  • GABA A R alpha 1
  • GABAAR alpha 1
  • GABAARa1
  • GABRA1
  • gamma-aminobutyric acid (GABA) A receptor, alpha 1
  • gamma-aminobutyric acid receptor subunit alpha-1

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 1 Antibody (NB300-191)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(2)
IP
(2)
WB
(7)
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Human
(1)
Mouse
(4)
Rat
(7)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB300-191 Applications Species
Bach EC, Morgan JW, Ewin SE et al. Chronic Ethanol Exposures Leads to a Negative Affective State in Female Rats That Is Accompanied by a Paradoxical Decrease in Ventral Hippocampus Excitability Frontiers in Neuroscience 2021-04-30 [PMID: 33994940] (WB) WB
Popoola DO, Nizhnikov ME, Cameron NM. Strain-specific programming of prenatal ethanol exposure across generations. Alcohol: An International Biomedical Journal 2017-01-03 [PMID: 28433421] (WB, Rat) WB Rat
Carlson SL, O'Buckley TK, Thomas R et al. Altered GABAA Receptor Expression and Seizure Threshold Following Acute Ethanol Challenge in Mice Lacking the RIIbeta Subunit of PKA. Neurochem Res. 2013-10-09 [PMID: 24104609] (WB, Mouse) WB Mouse
Mendu SK, Bhandage A, Jin Z, Birnir B. Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes PLoS One 2012-01-01 [PMID: 22927941] (ICC/IF, Rat, Human, Mouse) ICC/IF Rat, Human, Mouse
Lin TY, Lu CW, Wang CC et al. Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals Toxicol Appl Pharmacol 2012-07-01 [PMID: 22759588] (WB, Rat) WB Rat
Kumar S, Ren Q, Beckley JH, O'Buckley TK, Gigante ED, Santerre JL, Werner DF, Morrow AL. Ethanol Activation of Protein Kinase A Regulates GABA(A) Receptor Subunit Expression in the Cerebral Cortex and Contributes to Ethanol-Induced Hypnosis. Front Neurosci;6:44. 2012-01-01 [PMID: 22509146] (WB, Rat) WB Rat
Suryanarayanan A, Liang J, Meyer EM et al. Subunit Compensation and Plasticity of Synaptic GABA(A) Receptors Induced by Ethanol in ?4 Subunit Knockout Mice. Front Neurosci;5:110. 2011-01-01 [PMID: 21977012] (WB, Mouse) WB Mouse
Shen Y, Lindemeyer AK, Spigelman I et al. Plasticity of GABAA receptors after ethanol pre-exposure in cultured hippocampal neurons Mol Pharmacol 2011-03-01 [PMID: 21163967] (WB, Mouse) WB Mouse
Kumar S, Suryanarayanan A, Boyd KN et al. Ethanol reduces GABAA alpha1 subunit receptor surface expression by a protein kinase Cgamma-dependent mechanism in cultured cerebral cortical neurons. Mol Pharmacol;77(5):793-803. 2010-05-01 [PMID: 20159950] (ICC/IF, Rat) ICC/IF Rat
Marutha Ravindran CR, Mehta AK, Ticku MK. Effect of chronic administration of ethanol on the regulation of tyrosine kinase phosphorylation of the GABAA receptor subunits in the rat brain. Neurochem Res 2007-07-01 [PMID: 17404840] (IP, Rat) IP Rat
Show All 11 Publications.

Review for GABA-AR alpha 1 Antibody (NB300-191) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-191:
Filter by Applications
WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot GABA-AR alpha 1 NB300-191
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5
reviewed by:
Daniel Popoola
WB Rat 03/21/2017
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Summary

ApplicationWestern Blot
Sample Testedbrain and spinal cord,brain
SpeciesRat

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GABA-AR alpha 1 Antibody (NB300-191). (Showing 1 - 1 of 1 FAQs).

  1. Are there neutralizing peptides for this antibody?
    • There is no neutralizing peptide available for this antibody.

Secondary Antibodies

 

Isotype Controls

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Research Areas for GABA-AR alpha 1 Antibody (NB300-191)

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Recent Reviews

5
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1
4
0
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2
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1
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Daniel Popoola
03/21/2017
Application: WB
Species: Rat

Bioinformatics

Gene Symbol GABRA1
Entrez
Uniprot