GABA-A R beta 3 Antibody

Images

 
Western Blot: GABA-A R beta 3 Antibody [NB300-199] - 5-7 ug of mouse cerebellum lysates from wild type (Control) and beta 3 knockout (beta 3 K/O) animals showing specific immunolabeling of the ~53k beta 3-subunit of the ...read more
Simple Western: GABA-A R beta 3 Antibody [NB300-199] - Simple Western lane view shows a specific band for GABA-A Receptor Beta 3 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit

Order Details

GABA-A R beta 3 Antibody Summary

Immunogen
Fusion protein from the cytoplasmic loop of the beta 3-subunit of rat GABAA receptor
Specificity
Specific for the ~53k beta 3-subunit of the GABAA receptor in Western blots.
Clonality
Polyclonal
Host
Rabbit
Gene
GABRB3
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunohistochemistry Free-Floating
Application Notes
Use in Immunohistochemistry free-floating reported in scientific literature (PMID 26592770).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-199 in the following applications:

Publications
Read Publications using
NB300-199 in the following applications:

Alternate Names for GABA-A R beta 3 Antibody

  • ECA5
  • GABA(A) receptor beta-3 subunit
  • GABA(A) receptor subunit beta-3
  • GABA-A R beta 3
  • GABAA receptor beta-3 subunit
  • GABA-alpha receptor beta-2 subunit
  • GABAAR beta 3
  • GABAARb3
  • GABRB3
  • gamma-aminobutyric acid (GABA) A receptor, beta 3
  • gamma-aminobutyric acid receptor subunit beta-3
  • MGC9051

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC-FrFl

Publications for GABA-A R beta 3 Antibody (NB300-199)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IHC-FrFl, WB.


Filter By Application
ICC/IF
(1)
IHC-Fr
(2)
IHC-FrFl
(1)
WB
(3)
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Filter By Species
Human
(2)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB300-199 Applications Species
Nunes D, Kuner T. Disinhibition of olfactory bulb granule cells accelerates odour discrimination in mice. Nat Commun. 2015 Nov 23 [PMID:26592770] (WB, IHC-FrFl, Mouse) WB, IHC-FrFl Mouse
Du Y, Du Z, Zheng H, Wang D et al. GABA exists as a negative regulator of cell proliferation in spermaogonial stem cells. Cell Mol Biol Lett 2013 Feb 21 [PMID:23430456] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Lin TY, Lu CW, Wang CC et al. Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals Toxicol Appl Pharmacol 2012 Jul 1 [PMID:22759588] (WB, Rat) WB Rat
Fatemi SH, Folsom TD, KneelRE, Liesch SB. Metabotropic glutamate receptor 5 upregulation in children with autism is associated with underexpression of both Fragile X mental retardation protein and GABAA receptor beta 3 in adults with autism. Anat Rec (Hoboken) 2011 Oct [PMID:21901840] (WB, Human) WB Human
Fatemi SH, Reutiman TJ, Folsom TD et al. GABA(A) receptor downregulation in brains of subjects with autism. J Autism Dev Disord 2009 Feb [PMID:18821008] (Human) Human
Maison SF et al. Functional role of GABAergic innervation of the cochlea: phenotypic analysis of mice lacking GABA(A) receptor subunits alpha 1, alpha 2, alpha 5, alpha 6, beta 2, beta 3, or delta. J Neurosci 26:10315-26. 2006 [PMID:17021187] (IHC-Fr, Mouse) IHC-Fr Mouse
Slany, A et al. Rat beta 3 subunits expressed in human embryonic kidney 293 cells form high affinity [35-S]t-butylbicyclophosphorothionate binding sites modulated by several allosteric ligs of gamma-aminobutyric acid type A receptors. Mol. Pharmacol. 48: 385-91. 1995 [PMID:7565617]

Reviews for GABA-A R beta 3 Antibody (NB300-199) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB300-199:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot GABA-A R beta 3 NB300-199
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reviewed by:
Corey.
WB Mouse 04/04/2013
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Summary

ApplicationWestern Blot
Sample TestedMouse forebrain lysate
SpeciesMouse
LotWS112B
CommentsControls GABA A Receptor B3-Knockout homozygote mouse. Observed band at 55 kDa in Wt and het samples. No band observed for B3-KO homozygote mouse. 10% PVDF membrane was used in this experiment.

Blocking

Blocking DetailsOdyssey blocking buffer from Li-cor Bioscience

Primary Anitbody

Dilution Ratio1:300 in 1X PBS, O/N at 4C

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IgG
Secondary Manufacturer Cat#YE800CW
Secondary Concentration1:10,000

Details

Detection NotesOdyssey System

Comments

CommentsControls GABA A Receptor B3-Knockout homozygote mouse. Observed band at 55 kDa in Wt and het samples. No band observed for B3-KO homozygote mouse. 10% PVDF membrane was used in this experiment.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GABA-A R beta 3 Antibody (NB300-199). (Showing 1 - 3 of 3 FAQs).

  1. Could you please provide the immunogen sequence for Anti-GABA A Receptor beta 3 antibody and Anti-GABA A Receptor beta 2 antibody, catalog number NB300-199 and NB300-198 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived? Also, please let me know if this information can be made public. Thank you for your help in this matter.
    • The immunogen for NB300-199 falls within 340-420 amino acids, while the immunogen for NB300-198 falls within the range of 327-451.
  2. Which species is the sequence from?
    • This sequence came from rat.
  3. Could you please confirm the concentration for cat# NB300-199? The lot is WS712G.
    • The concentration of that lot # is 0.15 mg/mL.

Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol GABRB3
Entrez
Uniprot

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