GABA-A R beta 3 Antibody

Images

 
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Immunostaining of mouse retina showing specific labeling of the GABAA beta3 subunit in green, calbindin in red and DNA in blue.
Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Western blot of whole-olfactory bulb homogenates using the B3-subunit antibody in rAAV-Cre-injected versus non-injected GABRB3loxP/loxP mice (each six samples from ...read more
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Each channel was filtered frame by frame. The raw data was acquired at high confocal resolution (XY: 234.32 um x 234.32 um, pixel size = 0.114 um) using a 63x ...read more
Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Rat brain lysate showing specific immunolabeling of the 53 kDa Beta(3)-subunit of the GABA(A)-R
Immunohistochemistry-Paraffin: GABA-A R beta 3 Antibody [NB300-199] - Paraffin-embedded, formalin-fixed mouse head section. WB image submitted by a verified customer review.
Immunohistochemistry-Frozen: GABA-A R beta 3 Antibody [NB300-199] - Clustered distribution of the GABA-A R beta 3-subunit. Immunostaining using aG ABA-A R beta 3-subunit-selective antibody (red hot colour palette) in ...read more
Clustered distribution of the beta3-subunit.(a) Immunostaining using a beta3-subunit-selective antibody (red hot colour palette) in horizontal OB slices infected with rAAV-mGFP (grey). The images represent single ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GABA-A R beta 3 Antibody Summary

Immunogen
Fusion protein from the cytoplasmic loop of the beta 3 subunit. Accession # P63079
Specificity
Specific for the ~53 kDa beta 3-subunit of the GABAA receptor in Western blots.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABRB3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Simple Western reported by internal validation
  • Western Blot 1:1000
Application Notes
Use in Immunohistochemistry free-floating reported in scientific literature (PMID: 26592770). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17021187). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23430456). In Simple Western internal validation: Human Brain lysate at 0.5 mg/ml as sample; separated by size; antibody dilution of 1:100; observed molecular weight was 48 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-199 in the following applications:

Publications
Read Publications using
NB300-199 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 21901840).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/ml BSA, 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for GABA-A R beta 3 Antibody

  • ECA5
  • GABA A R beta 3
  • GABA(A) receptor beta-3 subunit
  • GABA(A) receptor subunit beta-3
  • GABAA receptor beta-3 subunit
  • GABA-alpha receptor beta-2 subunit
  • GABAAR beta 3
  • GABAARb3
  • GABRB3
  • gamma-aminobutyric acid (GABA) A receptor, beta 3
  • gamma-aminobutyric acid receptor subunit beta-3
  • MGC9051

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC

Publications for GABA-A R beta 3 Antibody (NB300-199)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
IHC-Fr
(2)
IHC-FrFl
(1)
WB
(8)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
Rat
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB300-199 Applications Species
Nwosu GI, Shen W, Zavalin K et al. GABAA Receptor ?3 Subunit Mutation N328D Heterozygous Knock-in Mice Have Lennox-Gastaut Syndrome International journal of molecular sciences 2023-05-08 [PMID: 37176165] (WB, Mouse)

Details:
Dilution: 1:1000
WB Mouse
Hernandez C, Shen Y, Hu N et al. GABRG2 Variants Associated with Febrile Seizures Biomolecules 2023-02-22 [PMID: 36979350] (WB, Human) WB Human
Michalettos G, Walter HL, Antunes ARP Et al. Effect of Anti-inflammatory Treatment with AMD3100 and CX3CR1 Deficiency on GABAA Receptor Subunit and Expression of Glutamate Decarboxylase Isoforms After Stroke Molecular neurobiology 2021-08-20 [PMID: 34417725] (WB, Mouse) WB Mouse
Hernandez C, Tian X, Hu N et al. Dravet syndrome associated mutations in GABRA1, GABRB2 and GABRG2 define the genetic landscape of defects of GABAA receptors Brain Commun 2021-06-07 [PMID: 34095830]
Chen M, Chen Y, Huo Q et al. Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models Molecular Neurodegeneration 2021-12-01 [PMID: 33663578]
Qu S, Zhou C, Howe R et al. The K328M substitution in the human GABAA receptor gamma2 subunit causes GEFS+ and premature sudden death in knock-in mice Neurobiology of disease 2021-02-11 [PMID: 33582225]
Qu S, Catron M, Zhou C et Al. GABAA receptor beta 3 subunit mutation D120N causes Lennox-Gastaut syndrome in knock-in mice Brain Commun 2020-05-30 [PMID: 32467926]
Bocker HT, Heinrich T, Liebmann L et al. The Na+/H+ Exchanger Nhe1 Modulates Network Excitability via GABA Release Cereb. Cortex 2018-12-12 [PMID: 30541023] (WB, Mouse) WB Mouse
Lu CY, Liu D, Jiang H et al. Effects of traumatic stress induced in the juvenile period on the expression of Gamma-aminobutyric acid receptor type A subunits in adult rat brain. Hindawi Neural Plasticity. 2017-03-02 [PMID: 28352479] (IF/IHC, WB, Rat) IF/IHC, WB Rat
Nunes D, Kuner T. Disinhibition of olfactory bulb granule cells accelerates odour discrimination in mice. Nat Commun. 2015-11-23 [PMID: 26592770] (WB, IHC-FrFl, Mouse) WB, IHC-FrFl Mouse
Show All 16 Publications.

Reviews for GABA-A R beta 3 Antibody (NB300-199) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB300-199:
Filter by Applications
IHC-P
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GABA-A R beta 3 NB300-199
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 08/22/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse brain
SpeciesMouse
LotKN905
Western Blot GABA-A R beta 3 NB300-199
Enlarge
5
reviewed by:
Corey .
WB Mouse 04/04/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse forebrain lysate
SpeciesMouse
LotWS112B

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GABA-A R beta 3 Antibody (NB300-199). (Showing 1 - 3 of 3 FAQs).

  1. Could you please provide the immunogen sequence for Anti-GABA A Receptor beta 3 antibody and Anti-GABA A Receptor beta 2 antibody, catalog number NB300-199 and NB300-198 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived? Also, please let me know if this information can be made public. Thank you for your help in this matter.
    • The immunogen for NB300-199 falls within 340-420 amino acids, while the immunogen for NB300-198 falls within the range of 327-451.
  2. Which species is the sequence from?
    • This sequence came from rat.
  3. Could you please confirm the concentration for cat# NB300-199? The lot is WS712G.
    • The concentration of that lot # is 0.15 mg/mL.

Secondary Antibodies

 

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
08/22/2018
Application: IHC-P
Species: Mouse

Corey .
04/04/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol GABRB3
Entrez
Uniprot