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KCNK3 Antibody (S374-48)

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Western Blot: KCNK3 Antibody (S374-48) [NBP2-42202] - Analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: 50 kDa.
Western Blot: KCNK3 Antibody (S374-48) [NBP2-42202] - Analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone ...read more
Immunocytochemistry/ Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TASK1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S374-48
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-42202AF405
Alexa Fluor 488
NBP2-42202AF488
Alexa Fluor 647
NBP2-42202AF647
Alexa Fluor 700
NBP2-42202AF700
Biotin
NBP2-42202B
DyLight 350
NBP2-42202UV
DyLight 405
NBP2-42202V
DyLight 488
NBP2-42202G
DyLight 550
NBP2-42202R
DyLight 650
NBP2-42202C
DyLight 680
NBP2-42202FR
DyLight 755
NBP2-42202IR
FITC
NBP2-42202F
HRP
NBP2-42202H
Janelia Fluor 549
NBP2-42202JF549
Janelia Fluor 646
NBP2-42202JF646
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KCNK3 Antibody (S374-48) Summary

Immunogen
Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). <30% identity with TASK3.
Localization
Membrane
Specificity
Less then 30% identity with TASK3.Detects approx 50kDa. Does not cross-react with TASK3.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KCNK3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
Application Notes
1 ug/ml of TASK1 Potassium Channel Antibody was sufficient for detection of TASK1 Potassium Channel in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for KCNK3 Antibody (S374-48)

  • Acid-sensitive potassium channel protein TASK-1
  • cardiac potassium channel
  • K2p3.1
  • OAT1
  • potassium channel subfamily K member 3
  • potassium channel, subfamily K, member 3
  • potassium inwardly-rectifying channel, subfamily K, member 3
  • TASK1
  • TASK-1
  • TASKacid-sensitive potassium channel protein TASK
  • TBAK1
  • TWIK-related acid-sensitive K(+) channel 1
  • TWIK-related acid-sensitive K+ channel
  • two P domain potassium channel
  • Two pore K(+) channel KT3.1
  • Two pore potassium channel KT3.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Other Available Formats

Alexa Fluor 405 NBP2-42202AF405
Alexa Fluor 488 NBP2-42202AF488
Alexa Fluor 647 NBP2-42202AF647
Alexa Fluor 700 NBP2-42202AF700
Biotin NBP2-42202B
DyLight 350 NBP2-42202UV
DyLight 405 NBP2-42202V
DyLight 488 NBP2-42202G
DyLight 550 NBP2-42202R
DyLight 650 NBP2-42202C
DyLight 680 NBP2-42202FR
DyLight 755 NBP2-42202IR
FITC NBP2-42202F
HRP NBP2-42202H
Janelia Fluor 549 NBP2-42202JF549
Janelia Fluor 646 NBP2-42202JF646
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Bioinformatics

Gene Symbol KCNK3
Uniprot