TREK 1 Antibody - BSA Free

Western Blot: TREK 1 Antibody [NB110-41535] - Detection of TREK 1 in membrane lysate fraction of human brain tissue.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF (-), IHC (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TREK 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TREK 1 Antibody - BSA Free (NB110-41535) is a polyclonal antibody validated for use in WB. Anti-TREK 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an N-terminal region of human TREK 1 (within residues 1-100). [Swiss-Prot# Q9NRT2]
• Localization: Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This TREK 1 antibody is useful inThis TREK 1 antibody is useful for Western Blot where a band is seen ~47 kDa. Please note that it is essential to run against membrane preps. This antibody is not recommended for Immunohistochemistry or Immunocytochemisrty.
• Control:

  TREK 1 Overexpression Lysate

Reactivity Notes

88% sequence identity with rat protein.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TREK 1 Antibody - BSA Free

• KCNK2
• MGC126742
• MGC126744
• potassium channel, subfamily K, member 2
• subfamily K, member 2
• tandem-pore-domain potassium channel TREK-1
• TREK 1
• TREK
• TREK-1 K(+) channel subunit
• TREK1
• TWIK-related potassium channel 1
• two-pore potassium channel 1

Background

TREK 1 (KCNK2 or two pore potassium channel TPKC1) belongs to two pore domain potassium channel (TC 1.A.1.8) family and it is a TWIK related K+ channel that acts as a background channel producing time/voltage-independent background current which drives the membrane potential toward the K+ equilibrium potential thereby affecting input resistance. TREK1 is characterized by mechanosensitivity, heat sensitivity, G-protein regulation, and activation by lipids, arachidonic acid, and volatile anesthetics such as chloroform, halothane and isoflurane. TREK1 is widely expressed in brain and genetic ablation has revealed involvement of this channel in depression, neuroprotection, pain perception, and ischemia-precipitated epilepsy. TREK1 gene inactivation produces mice with decreased sensitivity to volatile anesthetics, impaired PUFA-mediated neuroprotection and altered pain perception, depression-resistant phenotype and alteration in the efficacy of GPCR-associated cascade producing NO that leads to major endothelial dysfunction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREK 1 Antibody (NB110-41535)(1)

Publication using NB110-41535

• Zhang M, Yin HJ, Wang WP et al. Over-expressed human TREK-1 inhibits CHO cell proliferation via inhibiting PKA and p38 MAPK pathways and subsequently inducing G1 arrest. Acta Pharmacol Sin 2016-09-01 [PMID: 27397543] (WB)

Bioinformatics

• Gene Symbol: KCNK2