ASPA Antibody

Images

 
Western Blot: ASPA Antibody [NBP1-31754] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: ASPA Antibody [NBP1-31754] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aspartoacylase protein stained by partoacylase antibody [N1C3-2] diluted at ...read more
Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: ASPA Antibody [NBP1-31754] - Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ASPA Antibody [NBP1-31754] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ASPA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Aspartoacylase. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Porcine (95%), Bovine (93%), Monkey (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (85%), Rhesus Monkey (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASPA Antibody

  • ACY-2
  • ACY2aminoacylase 2
  • Aminoacylase-2
  • ASPaminoacylase-2
  • aspartoacylase (aminoacylase 2, Canavan disease)
  • aspartoacylase
  • EC 3.5.1.15

Background

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol ASPA