GABA-AR alpha 2 Antibody (S399-19) - BSA Free

Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 2 Antibody (D) Composite.

Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S399-19
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

GABA-AR alpha 2 Antibody (S399-19) - BSA Free Summary

• Description: Novus Biologicals Mouse GABA-AR alpha 2 Antibody (S399-19) - BSA Free (NBP2-59325) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GABA-AR alpha 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2
• Localization: Cytoplasm
• Specificity: Detects 55kDa. Does not cross-react with GABA-A Receptor Alpha1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABRA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Alternate Names for GABA-AR alpha 2 Antibody (S399-19) - BSA Free

• GABA A R alpha 2
• GABA(A) receptor subunit alpha-2
• GABAAR alpha 2
• GABAARa2
• GABRA2
• gamma-aminobutyric acid (GABA) A receptor, alpha 2
• gamma-aminobutyric acid receptor subunit alpha-2

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 2 Antibody (NBP2-59325)(1)

Publication using NBP2-59325

• Gawande DY, Shelkar GP, Narasimhan KKS et al. GluN2D subunit-containing NMDA receptors regulate reticular thalamic neuron function and seizure susceptibility Neurobiology of disease 2023-04-07 [PMID: 37031803] (WB, Mouse)

Bioinformatics

• Gene Symbol: GABRA2