Home » G2A/GPR132 » G2A Antibodies » G2A/GPR132 Antibody

G2A/GPR132 Antibody

 Submit a Review

Images

 
(
Enlarge)
Immunohistochemistry-Paraffin: G2A/GPR132 Antibody [NLS3702] - Analysis of anti-GPR132 / G2A antibody with human spleena at 3 ug/ml.
Immunohistochemistry-Paraffin: G2A/GPR132 Antibody [NLS3702] - Analysis of anti-GPR132 / G2A antibody with human spleena at 3 ug/ml.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

100% Guarantee on every product

G2A/GPR132 Antibody Summary

Immunogen
Synthetic 18 amino acid peptide from 3rd extracellular domain of human GPR132 / G2A.
Localization
Cell Membrane
Specificity
Human G2A/GPR132. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PRDM4 (61%).
Predicted Species
Monkey (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPR132
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3 - 28 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Gorilla (94%), Marmoset (83%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for G2A/GPR132 Antibody

  • G protein-coupled receptor 132
  • G2 accumulation protein
  • G2A
  • G2AG protein-coupled receptor G2A
  • GPR132
  • MGC99642
  • probable G-protein coupled receptor 132

Background

G2A belongs to the Lysophospholipid/Lysosphingolipid Receptor family and is an anti-proliferative cell cycle regulator that plays a critical role in controlling peripheral lymphocyte homeostasis. Loss of G2A expression leads to the development of a late-onset autoimmune syndrome (Le et al, 2001). Lysophosphatidylcholine (LPC) is the activating ligand for G2A. G2A is expressed in lymphoid tissues. ESTs have been isolated from normal human primary B-cell from tonsil, leukocyte, colon, lung, and pancreas/spleen, melanocyte/uterus/fetal heart libraries and from a human bone marrow cancer library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

IL-4 [Unconjugated]
6507-IL/CF
IL-4 [Unconjugated]
(3 Publications)
Species: Hu
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(19 Reviews)
(335 Publications)
Species: Hu
CD4 Antibody
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
(8 Reviews)
(29 Publications)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(22 Reviews)
(302 Publications)
Species: Hu
IL-10 [Biotin]
DY417
(6 Reviews)
(186 Publications)
Species: Mu

Acetylcholinesterase/A
NB100-1519
Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining of HepG2 (A) and Daudi cell lysates with antibody at 1 ug/mL (B) and HeLa (C) and Jurkat (D) cell lysates with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic and nuclear/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, PEP-ELISA, IHC-FrFl
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(33 Reviews)
(647 Publications)
Species: Hu
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(3 Reviews)
(1 Publication)
Species: Hu
CD8 Antibody
(53-6.7)
NBP1-49045
Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.
Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/-, and Asic2-/- mice at 45 days after immunization. Sections of the spinal cord anterior horn were stained with hematoxylin and eosin (E&H) to assess inflammation and immunostained for MCHII, CD4+, CD8+ infiltrating cells. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14302), licensed under a CC-BY licence.
Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
(2 Reviews)
(16 Publications)
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Proprotein
Convertase 7/PCSK7 An ...
NBP2-73621
Immunocytochemistry/Immunofluorescence: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PCSK7.
Flow Cytometry: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PCSK7 antibody, and then analyzed by flow cytometry.
Flow Cytometry: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PCSK7 antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: Flow, ICC/IF, CyTOF-ready
OGR1 Antibody
NLS1194
Immunohistochemistry-Paraffin: OGR1 Antibody [NLS1194] - Colon, Adenocarcinoma
Immunohistochemistry-Paraffin: OGR1 Antibody [NLS1194] - Analysis of brain, neurons and glia using this antibody at 2 ug/ ml.
Immunohistochemistry-Paraffin: OGR1 Antibody [NLS1194] - Analysis of human kidney.
Species: Hu, Mu, Rt, Po, Bv, Eq, Ha, Pm, Pm, Rb
Applications: ICC/IF, IHC, IHC-P
GPR4 Antibody
NLS145
Immunocytochemistry/Immunofluorescence: GPR4 Antibody [NLS145] - Analysis of anti-GPR4 antibody with HEK293 human embryonic kidney cells transfected with GPR4.
Immunohistochemistry-Paraffin: GPR4 Antibody [NLS145] - Brain Cortex Neurons and Glia
Immunocytochemistry/Immunofluorescence: GPR4 Antibody [NLS145] - Analysis of anti-GPR4 antibody with untransfected HEK293 human embryonic kidney cells.
Species: Hu, Ca, Pm, Pm
Applications: ICC/IF, IHC, IHC-P
IgG3 Antibody
(RM119) [Unconjugated
NBP2-62125
Immunohistochemistry-Paraffin: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody RM119 at a 1:2000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA
Immunohistochemistry: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - Immunohistochemistry of Human Lymphoid Tissue using NBP2-62125.
ELISA: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - ELISA of human immunoglobulins shows NBP2-62125 only reacts to human IgG3. No cross reactivity with other human immunoglobulins, mouse, rat, or goat IgG.
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, S-ELISA
IL-5 [HRP]
M5000
(3 Reviews)
(71 Publications)
Species: Mu
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(20 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
HLA DQ Antibody
(SPV-L3)
NBP2-45041
Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(1 Publication)
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF

Butyrylcholinesterase/ ...
AF9024
Western blot shows lysates of mouse heart tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Polyclonal Antibody (Catalog # AF9024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Butyrylcholinesterase/BCHE at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Butyrylcholinesterase/BCHE was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9024) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Species: Mu, Rt
Applications: WB, IHC
IL-6 [HRP]
D6050
(25 Reviews)
(466 Publications)
Species: Hu

Publications for G2A/GPR132 Antibody (NLS3702) (0)

There are no publications for G2A/GPR132 Antibody (NLS3702).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for G2A/GPR132 Antibody (NLS3702) (0)

There are no reviews for G2A/GPR132 Antibody (NLS3702). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for G2A/GPR132 Antibody (NLS3702): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for G2A/GPR132 Antibody (NLS3702) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional G2A/GPR132 Products

Bioinformatics Tool for G2A/GPR132 Antibody (NLS3702)

Discover related pathways, diseases and genes to G2A/GPR132 Antibody (NLS3702). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for G2A/GPR132 Antibody (NLS3702)

Discover more about diseases related to G2A/GPR132 Antibody (NLS3702).
 

Pathways for G2A/GPR132 Antibody (NLS3702)

View related products by pathway.

Research Areas for G2A/GPR132 Antibody (NLS3702)

Find related products by research area.

Blogs on G2A/GPR132

There are no specific blogs for G2A/GPR132, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  IHC Protocol
  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our G2A/GPR132 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol GPR132
Entrez
Uniprot