TDAG8/GPR65 Antibody - BSA Free

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil

Staining of human skeletal muscle shows low expression as expected.

Analysis in human tonsil and skeletal muscle tissues using NBP3-21356 antibody. Corresponding GPR65 RNA-seq data are presented for the same tissues.

Staining of human tonsil shows strong membranous positivity in non-germinal center cells.

Staining of human small intestinal shows strong membranous positivity in lymphoid cells and glandular cells,

Staining of human stomach shows strong membranous positivity in glandular cells and lymphoid cells

Staining of human skeletal muscle shows negative positivity in myocytes.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TDAG8/GPR65 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TDAG8/GPR65 Antibody - BSA Free (NBP3-21356) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LLIRCILEHAVNFEDHSNSGKRTYTMYRI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR65
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TDAG8/GPR65 Antibody - BSA Free

• G protein-coupled receptor 65
• GPR65
• G-protein coupled receptor 65
• Psychosine Receptor
• T-cell death-associated gene 8 protein
• TDAG8
• TDAG8hTDAG8

Background

GPR65 (TDAG8), a Lysophospholipid/Lysosphingolipid Receptor, is a molecular target for psychosine, a toxic lipid formed by the breakdown of galactosylceramide (cerebroside). A build-up of psychosine, caused by the lack of galactosylceramidase, results in the death of the myelin-synthesizing cells, oligodendrocytes, which is associated with Krabbe disease. GPR65 has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. ESTs have been isolated from human B-cell/lung/testis, brain/lung/testis, pancreas/spleen, lung/spleen, leukocyte, embryo, kidney, liver/spleen, thyroid, and vessel libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR65