Mouse IgG3 Isotype Control (133316) [Unconjugated]

NTera-2 human testicular embryonic carcinoma cell line wasstained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog #MAB1435,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB007,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog # F0102B).

• Reactivity: Mu
• Applications: Ctrl
• Clone: 133316
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Mouse IgG3 Isotype Control (133316) [Unconjugated] Summary

• Immunogen: KLH
• Specificity: Selected for use as a mouse IgG₃ isotype control. Recognizes KLH.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHG3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Control

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IgG3 Isotype Control (133316) [Unconjugated]

• DKFZp686H11213
• FLJ39988
• FLJ40036
• FLJ40253
• FLJ40587
• FLJ40789
• FLJ40834
• IgG3
• immunoglobulin heavy constant gamma 3 (G3m marker)
• MGC45809

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG3 Isotype Control (MAB007)(5)

Publications using MAB007

• A Stepanjuk, M Koel, M Pook, M Saare, K Jääger, M Peters, K Krjutškov, S Ingerpuu, A Salumets MUC20 expression marks the receptive phase of the human endometrium Reprod. Biomed. Online, 2019;0(0):. 2019-01-01 [PMID: 31519421] (IHC-P, Human)
• C Celik-Ozen Inhibition of poly(ADP-ribose) polymerase may have preventive potential for varicocoele-associated testicular damage in rats Andrology, 2016;0(0):. 2016-01-01 [PMID: 27992945] (IHC, Rat)
• Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R Resolvin E1 and chemokine-like receptor 1 mediate bone preservation. J Immunol, 2013;190(2):689-94. 2013-01-01 [PMID: 23241890] (ICC, Mouse)
• Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells. Mol Ther, 2013;21(1):228-39. 2013-01-01 [PMID: 23183535] (Flow Cytometry Control, Human)
• Martensson UE, Fenyo EM, Olde B, Owman C Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains. Virology, 2006;355(1):6-17. 2006-01-01 [PMID: 16904155] (Control, Human)

Bioinformatics

• Gene Symbol: IGHG3