G2A/GPR132 Antibody - BSA Free

Immunohistochemistry-Paraffin: G2A/GPR132 Antibody - BSA Free [NBP1-02347] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 11 ug/ml.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

G2A/GPR132 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from C-terminus of human G2A/GPR132.
• Epitope: C-Terminus
• Specificity: Human G2A/GPR132. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR132
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 12 ug/ml
• Application Notes: IHC: NBP1-02347was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (89%), Marmoset (89%), Gibbon (83%), Monkey (83%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for G2A/GPR132 Antibody - BSA Free

• G protein-coupled receptor 132
• G2 accumulation protein
• G2A
• G2AG protein-coupled receptor G2A
• GPR132
• MGC99642
• probable G-protein coupled receptor 132

Background

G2A belongs to the Lysophospholipid/Lysosphingolipid Receptor family and is an anti-proliferative cell cycle regulator that plays a critical role in controlling peripheral lymphocyte homeostasis. Loss of G2A expression leads to the development of a late-onset autoimmune syndrome (Le et al, 2001). Lysophosphatidylcholine (LPC) is the activating ligand for G2A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR132
• Uniprot:
  • Q9UNW8(Human)