Human Follistatin Recombinant Protein

Product Discontinued

Human Follistatin Recombinant Protein Summary

• Description: A single glycosylated polypeptide protein corresponding to amino acids Glu22 - Cys213 of IL2RA (P01589).
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Lyophilized from a 0.2 um filtered concentrated solution in Acetonitrile and TFA.
• Details of Functionality: The ED50, as determined by the inhibition of Activin A-mediated erythroid differentiation of human K562 cells, is less than 0.4 mg/mL.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: FST
• Purity: SDS-PAGE
• Endotoxin Note: Less than 1 EU/ug of rHuFollistatin 300 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with sterilized PBS to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human Follistatin Recombinant Protein

• follistatin isoform FST317
• Follistatin
• FS
• FSActivin-binding protein
• FST

Background

Follistatin binds with high affinity to Activin and BMP family members where it acts as an antagonist. Alternative splicing generates isoforms of 288 aa and 315 aa. Recombinant Follistatin 300is derived from human Follistatin 315 that includes a 15 aa. truncation at the C-terminus. Follistatin associates with cell surface heparan sulfate proteoglycans and regulates Activin clearance and bioavailability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FST