Recombinant Human Follistatin Protein

Product Discontinued

Recombinant Human Follistatin Protein Summary

• Description: Recombinant bioactive protein containing 288 amino acids for Human Follistatin
  
  

  Source:E. coli
  Amino Acid Sequence:(P19883)

• Details of Functionality: Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4 ug/ml in the presence of 7.5 ng/ml Activin A.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FST
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 31.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized from 10 mM Sodium phosphate (pH 7.5), 75 mM NaCl
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to a concentration of 0.1 - 1.0 ug/ul. The solution can then be diluted into other aqueous buffers andstored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Follistatin Protein

• follistatin isoform FST317
• Follistatin
• FS
• FSActivin-binding protein
• FST

Background

Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF-Beta ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-Beta 1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FST