Human Follistatin Recombinant Protein

Product Discontinued

Human Follistatin Recombinant Protein Summary

• Description: A biologically active protein corresponding to amino acids Gly30 - Asp329 of FST.
  
  

  Source: Spodoptera frugiperda, Sf 21 (baculovirus)

• Preparation Method: Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA.
• Details of Functionality: Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is typically 0.1 - 0.4 ug/mL in the presence of 7.5 ng/mL of rhActivin A.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: FST
• Purity: SDS-PAGE
• Endotoxin Note: <0.01 EU per 1 ug of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Reconstitute with 10 ug/ml in sterilized PBS containing at least 0.1% human or BSA to desired concentration.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human Follistatin Recombinant Protein

• follistatin isoform FST317
• Follistatin
• FS
• FSActivin-binding protein
• FST

Background

FST, also known as Follistatin, has a 344 amino acid long isoform that is 38 kDa and a short 317 amino acid that is 35 kDa, functions as an activin antagonist binding directly to it and inhibits the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). Studies of this protein are being performed in relation to polycystic ovary syndrome, pituitary adenoma, insulin resistance, Down syndrome, plasmacytoma, lymphocytic leukemia, pituitary tumor infertility, endometrial carcinoma, teratocarcinoma, azoospermia, meningitis, hepatocellular carcinoma, ovarian carcinoma, liver disease, endometriosis, prostate carcinoma, prostatitis, ovarian cancer, and adenoma. This protein has been also shown to have interactions with ANG, DIP2A, BMP4, FN1, and TXN in signal transduction activin A signaling regulation and TGF-beta signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FST