Home » Follistatin » Follistatin ELISA Kits » Rat Follistatin ELISA Kit (Colorimetric)

Rat Follistatin ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Rat Follistatin ELISA Kit (Colorimetric) [NBP3-08120] - Standard Curve Reference
ELISA: Rat Follistatin ELISA Kit (Colorimetric) [NBP3-08120] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

100% Guarantee on every product

Rat Follistatin ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 6.00
Intra-Assay
%CV < 6.00
Spike Recovery
83 - 105%
Kit Type
ELISA Kit (Colorimetric)
Gene
FST

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate (Dismountable) - 1 EA, Reference Standard - 2 vials, Concentrated Biotinylated Detection Ab (100X) - 120 uL, Concentrated HRP Conjugate (100X) - 120 uL, Reference Standard & Sample Diluent - 20 mL, Biotinylated Detection Ab Diluent - 14

Alternate Names for Rat Follistatin ELISA Kit (Colorimetric)

  • follistatin isoform FST317
  • Follistatin
  • FS
  • FSActivin-binding protein
  • FST

Background

FST, also known as Follistatin, has a 344 amino acid long isoform that is 38 kDa and a short 317 amino acid that is 35 kDa, functions as an activin antagonist binding directly to it and inhibits the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). Studies of this protein are being performed in relation to polycystic ovary syndrome, pituitary adenoma, insulin resistance, Down syndrome, plasmacytoma, lymphocytic leukemia, pituitary tumor infertility, endometrial carcinoma, teratocarcinoma, azoospermia, meningitis, hepatocellular carcinoma, ovarian carcinoma, liver disease, endometriosis, prostate carcinoma, prostatitis, ovarian cancer, and adenoma. This protein has been also shown to have interactions with ANG, DIP2A, BMP4, FN1, and TXN in signal transduction activin A signaling regulation and TGF-beta signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Activin A
[Unconjugated]
338-AC/CF
1 μg/lane of Recombinant Human/Mouse/Rat Activin A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at&amp;nbsp;14 kDa and 24 kDa, respectively.
Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC/CF) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 is 0.2‑1.2&amp;nbsp;ng/mL.
BG01V human embryonic stem cells were differentiated into endoderm using media supplemented with Recombinant Human/Mouse/Rat Activin A (Catalog&amp;nbsp;# 338-AC). Control cells were cultured in medium without recombinant Activin A. Differentiation into endoderm was confirmed by positive-staining for Claudin-6 (red) and Sox17 (green) using the Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog&amp;nbsp;#&amp;nbsp;MAB3656) and the Goat Anti-Human Sox17 Polyclonal Antibody (Catalog&amp;nbsp;#&amp;nbsp;AF1924), respectively. BG01V human embryonic stem cells are licensed from ViaCyte, Inc.
(492 Publications)
Species: Hu, Mu, Rt
Applications: BA
BRD2 Antibody
NBP1-30475
Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-30475] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (NBP1-30475). Detection: DAB
Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2 ug/mL) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.
Immunoprecipitation: BRD2 Antibody [NBP1-30475] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30474 and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (NBP2-76410). For blotting immunoprecipitated BRD2, NBP2-76410 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
GDF-8/Myostatin
[Unconjugated]
788-G8/CF
1 μg/lane of Recombinant Human/Mouse/Rat GDF-8/Myostatin was resolved withSDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by silver staining, showing&amp;nbsp;the main bands at 12&amp;nbsp;kDa and 24&amp;nbsp;kDa, respectively.
Recombinant Human/Mouse/Rat GDF-8/Myostatin (Catalog # 788-G8/CF) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 2-10 ng/mL.
(41 Publications)
Species: Hu, Mu, Rt
Applications: BA
TST Antibody
NBP1-87148
Immunohistochemistry-Paraffin: TST Antibody [NBP1-87148] - Staining of human colon shows high expression.
Immunohistochemistry-Paraffin: TST Antibody [NBP1-87148] - Staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: TST Antibody [NBP1-87148] - Staining in human colon and lymph node tissues using anti-TST antibody. Corresponding TST RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
BMP-4
[Unconjugated]
314-BP/CF
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.&amp;nbsp;
Recombinant Human BMP-4 (Catalog #314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 2.5-15 ng/mL.
(463 Publications)
Species: Hu
Applications: BA, BA
PLOD1 Antibody
NBP2-38770
Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p&lt;0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-251 MG and human cell line RT-4.
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.
(3 Publications)
Species: Hu, Mu
Applications: IHC-P, WB
IL-6 [HRP]
D6050
(700 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100&amp;nbsp;pg/mL.
(3 Reviews)
(739 Publications)
Species: Hu
Applications: BA
BDNF [HRP]
DBD00
(88 Publications)
Species: Hu
Applications: ELISA
POMC Antibody
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
(10 Publications)
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB

Follistatin-related Ge ...
DFLRG0
(8 Publications)
Species: Hu
Applications: ELISA
BMP-2
[Unconjugated]
355-BM/CF
Recombinant human/mouse/rat BMP-2 (355-BM/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 40-200 ng/mL.
(180 Publications)
Species: Hu, Mu, Rt
Applications: BA
Noggin
[Unconjugated]
6057-NG/CF
Recombinant Human Noggin (Catalog # 6057-NG/CF) inhibits BMP-4 induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The activity is approximately 30-fold greater than the top competitor's Noggin.
BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3 day incubation in recombinant human Noggin (25 µg/mL) from either R&amp;amp;D Systems (Lot 1, Lot 2; Catalog # 6057-NG) or from two separate competitors (Competitor&amp;nbsp;1, Competitor&amp;nbsp;2). Control cells were not incubated in Noggin (No Noggin). The cells were stained for the early ectoderm marker, Otx2, and the neuroectoderm marker, SOX1. (A) Representative images of SOX1 (green), Otx2 (red), and DAPI (blue) staining in embryonic stem cells differentiated with Noggin from R&amp;amp;D Systems or Noggin from Competitor 2. (B) SOX1+ clusters were quantified under each of the indicated culture conditions. Cells treated with R&amp;amp;D Systems Noggin showed an increase in SOX1+ cells compared to both untreated and competitor-treated cells. R&amp;amp;D Systems Noggin showed consistent differentiation across the lots tested. BG01V human embryonic stem cells are licensed from ViaCyte, Inc.
(171 Publications)
Species: Hu
Applications: BA
EGF [Unconjugated]
236-EG
1 µg/lane of Recombinant Human EGF was resolved&amp;nbsp;with SDS-PAGE&amp;nbsp; and visualized&amp;nbsp;by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
(669 Publications)
Species: Hu
Applications: BA
CS Citrate
Synthase Antibody (17
NBP2-43648
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Rat tissue extract (50ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] ) diluted at 1:1000.
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - A. 30 ug zebrafish heart lysate/extract 10 % SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (Negative) and transfected (Positive) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(16 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64&amp;nbsp;kDa (as indicated) using 1&amp;nbsp;µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) . This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog&amp;nbsp;# AF7094) and NorthernLights&amp;#8482; 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog&amp;nbsp;# NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(3 Reviews)
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
BMP-7
[Unconjugated]
354-BP/CF
Recombinant human BMP-7 (354-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.1-0.6 µg/mL.
(92 Publications)
Species: Hu
Applications: BA
Chordin
[Unconjugated]
758-CN/CF
Chordin [Unconjugated]
(11 Publications)
Species: Mu
Applications: BA
Rat Follistatin
ELISA Kit (Colorimetr ...
NBP3-08120
Rat Follistatin ELISA Kit (Colorimetric)
Species: Rt
Applications: ELISA

Publications for Follistatin ELISA Kit (NBP3-08120) (0)

There are no publications for Follistatin ELISA Kit (NBP3-08120).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Follistatin ELISA Kit (NBP3-08120) (0)

There are no reviews for Follistatin ELISA Kit (NBP3-08120). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Follistatin ELISA Kit (NBP3-08120) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Follistatin Products

Bioinformatics Tool for Follistatin ELISA Kit (NBP3-08120)

Discover related pathways, diseases and genes to Follistatin ELISA Kit (NBP3-08120). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Follistatin ELISA Kit (NBP3-08120)

Discover more about diseases related to Follistatin ELISA Kit (NBP3-08120).
 

Pathways for Follistatin ELISA Kit (NBP3-08120)

View related products by pathway.

PTMs for Follistatin ELISA Kit (NBP3-08120)

Learn more about PTMs related to Follistatin ELISA Kit (NBP3-08120).
 

Research Areas for Follistatin ELISA Kit (NBP3-08120)

Find related products by research area.

Blogs on Follistatin

There are no specific blogs for Follistatin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  Manual - Rat Follistatin ELISA Kit (Colorimetric)
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Rat Follistatin ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol FST