Follistatin Antibody (85918)

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Follistatin was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Follistatin Monoclonal Antibody (Catalog # MAB669) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
85918
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

Follistatin Antibody (85918) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Follistatin
Gly30-Asp329
Accession # P19883
Specificity
Detects human Follistatin in direct ELISAs and Western blots. It recognizes the 288 aa, 300 aa, and 315 aa isoforms of human Follistatin. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse Follistatin.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FST
Purity
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB669 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin Antibody (85918)

  • follistatin isoform FST317
  • Follistatin
  • FS
  • FSActivin-binding protein
  • FST

Background

Follistatin (FST) is a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa) protein with a 29 aa signal sequence, an N-terminal atypical TGF binding domain, three Follistatin domains that contain EGF-like and kazal-like motifs, and a highly acidic C-terminal tail. The first Follistatin domain (FS1) contains a heparin binding site, while FS1 and FS2 are most critical for activin binding and neutralization (3, 4). In addition to activin, Follistatin regulates bioavailability of many non-TGF-beta members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also regulates hematopoietic stem cell adhesion to fibronectin via FS2, and binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3 (3, 5, 6). Of three Follistatin isoforms, the full-length mature Follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative isoforms (5, 8, 9). The acidic tail is missing in the splice variant FST288 which shows the highest affinity for activins, while a partial tail exists in the proteolytically produced FST303, which shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa identity with mouse, rat, equine and ovine FST, 99% with porcine and 97% with bovine FST. Genetic deletion of Follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility (10).
  1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
  2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
  3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
  4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
  5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
  6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
  7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
  8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
  9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
  10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Follistatin Antibody (MAB669)(8)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Goat.

We have publications tested in 5 applications: ICC, IHC, IHC Frozen, IHC Paraffin-embedded, Neut.


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ICC
(3)
IHC
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(2)
Neut
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Rat
(1)
Canine
(1)
Goat
(1)
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Showing Publications 1 - 8 of 8.
Publications using MAB669 Applications Species
W Wu, XL Wu, YQ Ji, Z Gao Differentiation of nestin?negative human hair follicle outer root sheath cells into neurons in�vitro Mol Med Rep, 2017;0(0):. 2017 [PMID: 28534946] (ICC,, Human) ICC Human
Jiang, Ming, Ku, Wei-Yao, Zhou, Zhongren, Dellon, Evan S, Falk, Gary W, Nakagawa, Hiroshi, Wang, Mei-Lun, Liu, Kuancan, Wang, Jun, Katzka, David A, Peters, Jeffrey, Lan, Xiaopeng, Que, Jianwen BMP-driven NRF2 activation in esophageal basal cell differentiation and eosinophilic esophagitis. J Clin Invest, 2015;125(4):1557-68. 2015 [PMID: 25774506] (IHC,, Mouse) IHC Mouse
Gao X, Wei S, Lai K Nucleolar follistatin promotes cancer cell survival under glucose-deprived conditions through inhibiting cellular rRNA synthesis. J. Biol. Chem., 2010;285(47):36857-64. 2010 [PMID: 20843798] (ICC,, Human) ICC Human
Kobayashi T, Iwasaki T, Amagai M Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells. J. Invest. Dermatol., 2010;130(8):1988-95. 2010 [PMID: 20357816] (IHC Frozen,, Canine) IHC Frozen Canine
O'Neill JP, Velalar CN, Lee DI, Zhang B, Nakanishi T, Tang Y, Selaru F, Ross D, Meltzer SJ, Hussain A Thapsigargin resistance in human prostate cancer cells. Cancer, 2006;107(3):649-59. 2006 [PMID: 16770788] (ICC,, Human) ICC Human
Silva JR, Tharasanit T, Taverne MA, van der Weijden GC, Santos RR, Figueiredo JR, van den Hurk R The activin-follistatin system and in vitro early follicle development in goats. J. Endocrinol., 2006;189(1):113-25. 2006 [PMID: 16614386] (IHC Paraffin-embedded,, Goat) IHC Paraffin-embedded Goat
Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts. Arthritis Rheum., 2004;50(8):2521-30. 2004 [PMID: 15334466] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Abe Y, Abe T, Aida Y, Hara Y, Maeda K Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells. J. Bone Miner. Res., 2004;19(8):1302-7. 2004 [PMID: 15231018] (Neut,, Rat) Neut Rat

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Bioinformatics

Gene Symbol FST
Uniprot