FLI1 Antibody

Images

 
Western Blot: FLI1 Antibody [NBP2-16500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: FLI1 Antibody [NBP2-16500] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLI1 protein stained by FLI1 antibody diluted at 1:500. Blue: Hoechst 33343 ...read more
Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.
Western Blot: FLI1 Antibody [NBP2-16500] - Sample (30 ug of whole cell lysate) A: Raji B: THP-1 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: FLI1 Antibody [NBP2-16500] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGE FLI1 antibody dilution: 1:1000
Western Blot: FLI1 Antibody [NBP2-16500] - Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with FLI1 antibody diluted by 1:1000
Western Blot: FLI1 Antibody [NBP2-16500] - A. 30 ug BCL-1 whole cell lysate/extract 10 % SDS-PAGEFLI1 antibody dilution: 1:1000
Immunoprecipitation: FLI1 Antibody [NBP2-16500] - IP samples: Raji whole cell extract A. 40 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 ug FLI1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.95 mg/ml

Order Details

FLI1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.95 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FLI1 Antibody

  • ERGB
  • EWSR2
  • FLI1
  • Friend leukemia integration 1 transcription factor
  • Friend leukemia virus integration 1
  • Proto-oncogene Fli-1
  • SIC-1
  • Transcription factor ERGB

Background

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLI1
Entrez
Uniprot