ETS2 Antibody

Images

 
Western Blot: ETS2 Antibody [NBP2-14951] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ETS2 Antibody [NBP2-14951] - Paraformaldehyde-fixed HeLa, using ETS2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) ...read more
Immunohistochemistry-Paraffin: ETS2 Antibody [NBP2-14951] - Paraffin-embedded rat fore brain. ETS2 antibody dilution: 1:500.
Chromatin Immunoprecipitation: ETS2 Antibody [NBP2-14951] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by ...read more
Immunoprecipitation: ETS2 Antibody [NBP2-14951] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 ug of ...read more

Product Details

Summary
Reactivity Hu, Rt, BvSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ETS2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ETS2. The exact sequence is proprietary.
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ETS2 Antibody

  • ETS2IT1
  • human erythroblastosis virus oncogene homolog 210protein C-ets-2
  • oncogene ETS-2
  • v-ets avian erythroblastosis virus E2 oncogene homolog 2
  • v-ets avian erythroblastosis virus E26 oncogene homolog 2
  • v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)

Background

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ETS2 Antibody (NBP2-14951) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETS2
Uniprot