FLI1 Antibody (S01-0C2)

Western Blot: FLI1 Antibody (S01-0C2) [NBP3-19635] - Western blot detection of FLI1 in Jurkat, C6 cell lysates using NBP3-19635 (1:500 diluted). Predicted band size: 51kDa. Observed band size: 51kDa.

Immunohistochemistry-Paraffin: FLI1 Antibody (S01-0C2) [NBP3-19635] - Immunohistochemistry of FLI1 in paraffin-embedded Human lung cancer tissue using NBP3-19635 at dilution 1/50

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: S01-0C2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

FLI1 Antibody (S01-0C2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human FLI1 (Uniprot # Q01543)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FLI1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for FLI1 Antibody (S01-0C2)

• ERGB
• EWSR2
• FLI1
• Friend leukemia integration 1 transcription factor
• Friend leukemia virus integration 1
• Proto-oncogene Fli-1
• SIC-1
• Transcription factor ERGB

Background

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLI1