Recombinant Human FIH-1/HIF-1AN Protein

SDS-Page: Recombinant Human FIH-1/HIF-1AN Protein [NBC1-22596] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 40.2 kDa (349 aa), confirmed by MALDI-TOF. Concentration: 1 mg/ml (determined by Bradford assay)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human FIH-1/HIF-1AN Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-349 of Human FIH-1/HIF-1AN
  
  

  Source: E.coli

  Amino Acid Sequence: MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HIF1AN
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 40.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human FIH-1/HIF-1AN Protein

• DKFZp762F1811
• EC 1.14.11.16
• factor inhibiting HIF1
• Factor inhibiting HIF-1
• FIH1
• FIH-1
• FIH1FIH-1
• FLJ20615
• FLJ22027
• HIF1AN
• HIF-1AN
• hypoxia inducible factor 1, alpha subunit inhibitor
• hypoxia-inducible factor 1-alpha inhibitor
• Hypoxia-inducible factor asparagine hydroxylase
• Peptide-aspartate beta-dioxygenase

Background

Description: Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1alpha C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1alpha transactivation domain results in blockage of the HIF1alpha-p300/CBP interaction and represses transcriptional activity of HIF1alpha-targeted genes. Recombinant human HIF1AN protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HIF1AN