Fibromodulin/FMOD Antibody - BSA Free

Western Blot: Fibromodulin/FMOD Antibody [NBP2-16494] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 10% SDS PAGE Fibromodulin antibody diluted at 1:1000

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Fibromodulin/FMOD Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Fibromodulin/FMOD. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Mouse (96%), Rat (96%), Rhesus Macaque (99%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FMOD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Fibromodulin/FMOD Antibody - BSA Free

• Collagen-binding 59 kDa protein
• fibromodulin proteoglycan
• Fibromodulin
• FM
• FMOD
• Keratan sulfate proteoglycan fibromodulin
• SLRR2E
• SLRR2EKSPG fibromodulin

Background

Fibromodulin is a member of a family of small interstitial proteoglycans, containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. It may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Fibromodulin/FMOD Antibody (NBP2-16494)(1)

Publication using NBP2-16494

• Herchenhan A, Uhlenbrock F, Eliasson P et al. Lysyl Oxidase activity is required for ordered collagen fibrillogenesis by tendon cells. J. Biol. Chem. 2015-05-15 [PMID: 25979340] (WB, Human)
  
  Details:
  Tenascin X antibody was used at 1:500 dilution with ON 4C incubation for WB detection of established collagen fibrillogenesis regulator Tenascin X protein on samples from the control or beta-aminoproprionitrile /BAPN treated tendon constructs which were prepared using tendon fibroblasts isolated from human semitendinosus and gracilis tendon of human anterior cruciate ligament/ACL rupture patients undergoing reconstructive surgery. See the full text for protocol related details and the data is shown in FIGURE 7A.

Bioinformatics

• Gene Symbol: FMOD
• Uniprot:
  • Q06828()