FGFR4 Antibody (JE59-96)

Western Blot: FGFR4 Antibody (JE59-96) [NBP3-32340] - Western blot analysis of FGFR4 on different lysates with Rabbit anti-FGFR4 antibody (NBP3-32340) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: SW480 cell lysate Lane 4: Raji cell lysate Lane 5: PANC-1 cell lysate Lane 6: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 88 kDa Observed band size: 88/110 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32340) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: FGFR4 Antibody (JE59-96) [NBP3-32340] - Flow cytometric analysis of Caco-2 cells labeling FGFR4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32340, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow
• Clone: JE59-96
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FGFR4 Antibody (JE59-96) Summary

• Description: Novus Biologicals Rabbit FGFR4 Antibody (JE59-96) (NBP3-32340) is a recombinant monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human FGFR4 aa 753-802 / 802. (Uniprot: P22455)
• Localization: Cell membrane, Endosome, Endoplasmic reticulum; Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FGFR4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Western Blot 1:1000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FGFR4 Antibody (JE59-96)

• CD334 antigen
• CD334
• EC 2.7.10
• EC 2.7.10.1
• FGF R4
• FGFR4
• FGFR-4
• fibroblast growth factor receptor 4
• JTK2hydroxyaryl-protein kinase
• MGC20292
• protein-tyrosine kinase
• TKF
• tyrosine kinase related to fibroblast growth factor receptor
• tyrosylprotein kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGFR4