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FGFR3 Antibody (JM110-33)

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Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of FGFR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: 293 cell lysateLane 4: MCF-7 cell lysate
Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of FGFR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary ...read more
Immunocytochemistry/ Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse testis tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.
Flow Cytometry: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of A549 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Immunocytochemistry/ Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse brain tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JM110-33
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

100% Guarantee on every product

FGFR3 Antibody (JM110-33) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human FGFR3 aa 30-63 / 806. (SwissProt: P22607 Human; SwissProt: Q61851 Mouse; Unigene:23671 Rat)
Localization
Cell membrane, Endoplasmic reticulum, Cytoplasmic vesicle, Secreted.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FGFR3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000
Publications
Read Publication using
NBP2-66840 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for FGFR3 Antibody (JM110-33)

  • CD333
  • CEK
  • EC 2.7.10
  • FGF R3
  • FGFR3
  • fibroblast growth factor receptor 3
  • HSFGFR3EX
  • JTK4
  • JTK4thanatophoric dwarfism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: FGFR2 Antibody (5C5) [NBP2-00932] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2.
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FGFR3 Antibody
(JM110-33)
NBP2-66840
FGFR3 Antibody (JM110-33)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for FGFR3 Antibody (NBP2-66840)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol FGFR3