232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

710-MF) inhibits Recom-binant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human FGF acidic (0.3 ng/mL) activity elicited by Recom-binant Mouse FGF R3 (IIIc) Fc Chimera (12 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse FGF R3 Mono-clonal Antibody (Catalog # MAB710). The ND₅₀ is typically 3-12 µg/mL in the presence of heparin (10 µg/mL)." class="big_lightbox"> 710-MF) inhibits Recom-binant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human FGF acidic (0.3 ng/mL) activity elicited by Recom-binant Mouse FGF R3 (IIIc) Fc Chimera (12 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse FGF R3 Mono-clonal Antibody (Catalog # MAB710). The ND₅₀ is typically 3-12 µg/mL in the presence of heparin (10 µg/mL)." alt="Recombinant Mouse FGF R3 (IIIc) Fc Chimera (Catalog # 710-MF) inhibits Recom-binant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human FGF acidic (0.3 ng/mL) activity elicited by Recom-binant Mouse FGF R3 (IIIc) Fc Chimera (12 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse FGF R3 Mono-clonal Antibody (Catalog # MAB710). The ND₅₀ is typically 3-12 µg/mL in the presence of heparin (10 µg/mL)." class="big_thumb" />

1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND₅₀ is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL)." class="big_lightbox"> 1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND₅₀ is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL)." alt="Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND₅₀ is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL)." class="big_thumb" />

AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Biotinylated Recombinant Human FGFR3 (IIIb) His-tag Avi-tag Protein (Catalog # AVI11136) binds with an ED₅₀ of 1.00-6.00 µg/mL." class="big_lightbox"> AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Biotinylated Recombinant Human FGFR3 (IIIb) His-tag Avi-tag Protein (Catalog # AVI11136) binds with an ED₅₀ of 1.00-6.00 µg/mL." alt="In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Biotinylated Recombinant Human FGFR3 (IIIb) His-tag Avi-tag Protein (Catalog # AVI11136) binds with an ED₅₀ of 1.00-6.00 µg/mL." class="big_thumb" />

AF232) coated plate, in the presence of 50 ng/mL of Recombinant Human FGFacidic/FGF1 (232-FA), Recombinant Human FGFR3 alpha (IIIb) Fc Chimera Avi-tag Protein (Catalog # AVI11029) binds with an ED₅₀ of 40.0-240 ng/mL." class="big_lightbox"> AF232) coated plate, in the presence of 50 ng/mL of Recombinant Human FGFacidic/FGF1 (232-FA), Recombinant Human FGFR3 alpha (IIIb) Fc Chimera Avi-tag Protein (Catalog # AVI11029) binds with an ED₅₀ of 40.0-240 ng/mL." alt="In a Human FGFacidic/FGF1 antibody (AF232) coated plate, in the presence of 50 ng/mL of Recombinant Human FGFacidic/FGF1 (232-FA), Recombinant Human FGFR3 alpha (IIIb) Fc Chimera Avi-tag Protein (Catalog # AVI11029) binds with an ED₅₀ of 40.0-240 ng/mL." class="big_thumb" />

232-FA) with a ED₅₀ of 0.250-2.50 µg/mL" class="big_lightbox"> 232-FA) with a ED₅₀ of 0.250-2.50 µg/mL" alt="Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human FGFR3 Fc Chimera Avi-tag Protein (Catalog # AVI766) binds to Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein (232-FA) with a ED₅₀ of 0.250-2.50 µg/mL" class="big_thumb" />

232-FA) with an ED₅₀ of 0.700-7.00 μg/mL." class="big_lightbox"> 232-FA) with an ED₅₀ of 0.700-7.00 μg/mL." alt="Measured by its binding ability in a functional ELISA. Recombinant Human FGFR3 alpha (IIIb) His-tag Protein (Catalog # 11136-FR) binds to Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein (232-FA) with an ED₅₀ of 0.700-7.00 μg/mL." class="big_thumb" />