FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Images

 
Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution
Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF.
Immunohistochemistry: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody.
Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - FGF-2 antibody ( 3D9 ) at 1:1000 + Recombinant human bFGF.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FGF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
Theoretical MW
30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGF basic/FGF2/bFGF Antibody (NBP2-70718). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70718AF350
Alexa Fluor 405 NBP2-70718AF405
Alexa Fluor 488 NBP2-70718AF488
Alexa Fluor 532 NBP2-70718AF532
Alexa Fluor 594 NBP2-70718AF594
Alexa Fluor 647 NBP2-70718AF647
Alexa Fluor 700 NBP2-70718AF700
Alexa Fluor 750 NBP2-70718AF750
Biotin NBP2-70718B
DyLight 350 NBP2-70718UV
DyLight 405 NBP2-70718V
DyLight 488 NBP2-70718G
DyLight 550 NBP2-70718R
DyLight 594 NBP2-70718DL594
DyLight 650 NBP2-70718C
DyLight 680 NBP2-70718FR
DyLight 755 NBP2-70718IR
FITC NBP2-70718F
HRP NBP2-70718H
Janelia Fluor 549 NBP2-70718JF549
Janelia Fluor 646 NBP2-70718JF646

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Bioinformatics

Gene Symbol FGF2