Human FGF-4 ELISA Kit (Colorimetric)

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ELISA: Human FGF-4 ELISA Kit (Colorimetric) [KA1712] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Lysate, Tissue Lysate
Sensitivity
50 pg/mL

Order Details

Human FGF-4 ELISA Kit (Colorimetric) Summary

Sensitivity
50 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FGF4

Applications/Dilutions

Application Notes
FGF4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF4.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. HRP-Streptavidin Concentrate (Item G): 8 µl 6,000x concentrated HRP-conjugated streptavidin.
  2. FGF-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human FGF-4.
  3. Detection Antibody FGF-4 (Item F): 2 vial of biotinylated anti-human FGF-4 (each vial is enough to assay half microplate).
  4. Cell lysate buffer (Item J): 5 ml 2x cell lysate buffer.
  5. Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell lysate/tissuelysate/reagent) diluent.
  6. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  7. Standards (Item C): 2 vials of recombinant human FGF-4.
  8. Sample Diluent Buffer (Item D): 10 ml of 5x concentrated buffer. For Standard/Sample (cell lysate/tissue lysate) diluent.
  9. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  10. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human FGF-4 ELISA Kit (Colorimetric)

  • FGF4
  • FGF-4
  • fibroblast growth factor 4
  • HBGF-4
  • HBGF-4Transforming protein KS3
  • heparin secretory transforming protein 1
  • Heparin secretory-transforming protein 1
  • Heparin-binding growth factor 4
  • HST-1
  • HST-1HSTF-1
  • HSTF1fibroblast growth factor 4 splice isoform
  • HSTFGF-4
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • KFGF
  • K-FGF
  • KS3
  • oncogene HST

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FGF4
Entrez